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Meinrad Busslinger

Acquired immunity to foreign pathogens depends on the differentiation of B and T lymphocytes from hematopoietic stem cells, which is controlled by a multitude of transcription factors. We are interested in understanding the process by which transcription factors regulate the commitment of early hematopoietic progenitors to the B cell lineage and control their subsequent differentiation to mature B cells and plasma cells. We investigate the underlying transcriptional control mechanisms by using mouse transgenic, cell biological, and genome-wide molecular approaches.

Publications

Fujimura, N., Kuzelova, A., Ebert, A., Strnad, H., Lachova, J., Machon, O., Busslinger, M., Kozmik, Z. (2018) Polycomb repression complex 2 is required for the maintenance of retinal progenitor cells and balanced retinal differentiation. Dev Biol. 433(1):47-60
Gu, YF., Cohn, S., Christie, A., McKenzie, T., Wolff, N., Do, QN., Madhuranthakam, AJ., Pedrosa, I., Wang, T., Dey, A., Busslinger, M., Xie, XJ., Hammer, RE., McKay, RM., Kapur, P., Brugarolas, J. (2017) Modeling Renal Cell Carcinoma in Mice: Bap1 and Pbrm1 Inactivation Drive Tumor Grade. Cancer Discov. 7(8):900-917
Kreslavsky, T., Vilagos, B., Tagoh, H., Poliakova, DK., Schwickert, TA., Wöhner, M., Jaritz, M., Weiss, S., Taneja, R., Rossner, MJ., Busslinger, M. (2017) Essential role for the transcription factor Bhlhe41 in regulating the development, self-renewal and BCR repertoire of B-1a cells. Nat Immunol. 18(4):442-455
Smeenk, L., Fischer, M., Jurado, S., Jaritz, M., Azaryan, A., Werner, B., Roth, M., Zuber, J., Stanulla, M., den Boer, ML., Mullighan, CG., Strehl, S., Busslinger, M. (2017) Molecular role of the PAX5-ETV6 oncoprotein in promoting B-cell acute lymphoblastic leukemia. EMBO J. 36(6):718-735
Adams, WC., Chen, YH., Kratchmarov, R., Yen, B., Nish, SA., Lin, WW., Rothman, NJ., Luchsinger, LL., Klein, U., Busslinger, M., Rathmell, JC., Snoeck, HW., Reiner, SL. (2016) Anabolism-Associated Mitochondrial Stasis Driving Lymphocyte Differentiation over Self-Renewal. Cell Rep. 17(12):3142-3152
Ali, AK., Oh, JS., Vivier, E., Busslinger, M., Lee, SH. (2016) NK Cell-Specific Gata3 Ablation Identifies the Maturation Program Required for Bone Marrow Exit and Control of Proliferation. J Immunol. 196(4):1753-67
Delconte, RB., Shi, W., Sathe, P., Ushiki, T., Seillet, C., Minnich, M., Kolesnik, TB., Rankin, LC., Mielke, LA., Zhang, JG., Busslinger, M., Smyth, MJ., Hutchinson, DS., Nutt, SL., Nicholson, SE., Alexander, WS., Corcoran, LM., Vivier, E., Belz, GT., Carotta, S., Huntington, ND. (2016) The Helix-Loop-Helix Protein ID2 Governs NK Cell Fate by Tuning Their Sensitivity to Interleukin-15. Immunity. 44(1):103-15
Kollmann, K., Heller, G., Schneckenleithner, C., Warsch, W., Scheicher, R., Ott, RG., Schäfer, M., Fajmann, S., Schlederer, M., Schiefer, AI., Reichart, U., Mayerhofer, M., Hoeller, C., Zöchbauer-Müller, S., Kerjaschki, D., Bock, C., Kenner, L., Hoefler, G., Freissmuth, M., Green, AR., Moriggl, R., Busslinger, M., Malumbres, M., Sexl, V. (2016) A Kinase-Independent Function of CDK6 Links the Cell Cycle to Tumor Angiogenesis. Cancer Cell. 30(2):359-360
Leong, YA., Chen, Y., Ong, HS., Wu, D., Man, K., Deleage, C., Minnich, M., Meckiff, BJ., Wei, Y., Hou, Z., Zotos, D., Fenix, KA., Atnerkar, A., Preston, S., Chipman, JG., Beilman, GJ., Allison, CC., Sun, L., Wang, P., Xu, J., Toe, JG., Lu, HK., Tao, Y., Palendira, U., Dent, AL., Landay, AL., Pellegrini, M., Comerford, I., McColl, SR., Schacker, TW., Long, HM., Estes, JD., Busslinger, M., Belz, GT., Lewin, SR., Kallies, A., Yu, D. (2016) CXCR5(+) follicular cytotoxic T cells control viral infection in B cell follicles. Nat Immunol. 17(10):1187-96
Mackay, LK., Minnich, M., Kragten, NA., Liao, Y., Nota, B., Seillet, C., Zaid, A., Man, K., Preston, S., Freestone, D., Braun, A., Wynne-Jones, E., Behr, FM., Stark, R., Pellicci, DG., Godfrey, DI., Belz, GT., Pellegrini, M., Gebhardt, T., Busslinger, M., Shi, W., Carbone, FR., van Lier, RA., Kallies, A., van Gisbergen, KP. (2016) Hobit and Blimp1 instruct a universal transcriptional program of tissue residency in lymphocytes. Science. 352(6284):459-63
Minnich, M., Tagoh, H., Bönelt, P., Axelsson, E., Fischer, M., Cebolla, B., Tarakhovsky, A., Nutt, SL., Jaritz, M., Busslinger, M. (2016) Multifunctional role of the transcription factor Blimp-1 in coordinating plasma cell differentiation. Nat Immunol. 17(3):331-43
Pang, SH., Minnich, M., Gangatirkar, P., Zheng, Z., Ebert, A., Song, G., Dickins, RA., Corcoran, LM., Mullighan, CG., Busslinger, M., Huntington, ND., Nutt, SL., Carotta, S. (2016) PU.1 cooperates with IRF4 and IRF8 to suppress pre-B-cell leukemia. Leukemia. 30(6):1375-87
Pasquarella, A., Ebert, A., Pereira de Almeida, G., Hinterberger, M., Kazerani, M., Nuber, A., Ellwart, J., Klein, L., Busslinger, M., Schotta, G. (2016) Retrotransposon derepression leads to activation of the unfolded protein response and apoptosis in pro-B cells. Development. 143(10):1788-99
Tellier, J., Shi, W., Minnich, M., Liao, Y., Crawford, S., Smyth, GK., Kallies, A., Busslinger, M., Nutt, SL. (2016) Blimp-1 controls plasma cell function through the regulation of immunoglobulin secretion and the unfolded protein response. Nat Immunol. 17(3):323-30
Valent, P., Groner, B., Schumacher, U., Superti-Furga, G., Busslinger, M., Kralovics, R., Zielinski, C., Penninger, JM., Kerjaschki, D., Stingl, G., Smolen, JS., Valenta, R., Lassmann, H., Kovar, H., Jäger, U., Kornek, G., Müller, M., Sörgel, F. (2016) Paul Ehrlich (1854-1915) and His Contributions to the Foundation and Birth of Translational Medicine. J Innate Immun. 8(2):111-20
Wöhner, M., Tagoh, H., Bilic, I., Jaritz, M., Poliakova, DK., Fischer, M., Busslinger, M. (2016) Molecular functions of the transcription factors E2A and E2-2 in controlling germinal center B cell and plasma cell development. J Exp Med. 213(7):1201-21
Ebert, A., Hill, L., Busslinger, M. (2015) Spatial Regulation of V-(D)J Recombination at Antigen Receptor Loci. Adv Immunol. 128:93-121
Fenton, RA., Poulsen, SB., de la Mora Chavez, S., Soleimani, M., Busslinger, M., Dominguez Rieg, JA., Rieg, T. (2015) Caffeine-induced diuresis and natriuresis is independent of renal tubular NHE3. Am J Physiol Renal Physiol. 308(12):F1409-20
Nutt, SL., Heavey, B., Rolink, AG., Busslinger, M. (2015) Pillars Article: Commitment to the B-lymphoid lineage depends on the transcription factor Pax5. Nature. 1999. 401: 556-562. J Immunol. 195(3):766-72
Ohnmacht, C., Park, JH., Cording, S., Wing, JB., Atarashi, K., Obata, Y., Gaboriau-Routhiau, V., Marques, R., Dulauroy, S., Fedoseeva, M., Busslinger, M., Cerf-Bensussan, N., Boneca, IG., Voehringer, D., Hase, K., Honda, K., Sakaguchi, S., Eberl, G. (2015) MUCOSAL IMMUNOLOGY. The microbiota regulates type 2 immunity through RORγt⁺ T cells. Science. 349(6251):989-93
Witkowski, MT., Cimmino, L., Hu, Y., Trimarchi, T., Tagoh, H., McKenzie, MD., Best, SA., Tuohey, L., Willson, TA., Nutt, SL., Busslinger, M., Aifantis, I., Smyth, GK., Dickins, RA. (2015) Activated Notch counteracts Ikaros tumor suppression in mouse and human T-cell acute lymphoblastic leukemia. Leukemia. 29(6):1301-11
Yamano, T., Nedjic, J., Hinterberger, M., Steinert, M., Koser, S., Pinto, S., Gerdes, N., Lutgens, E., Ishimaru, N., Busslinger, M., Brors, B., Kyewski, B., Klein, L. (2015) Thymic B Cells Are Licensed to Present Self Antigens for Central T Cell Tolerance Induction. Immunity. 42(6):1048-61
Busslinger, M., Tarakhovsky, A. (2014) Epigenetic control of immunity. Cold Spring Harb Perspect Biol. 6(6)
Jurkin, J., Henkel, T., Nielsen, AF., Minnich, M., Popow, J., Kaufmann, T., Heindl, K., Hoffmann, T., Busslinger, M., Martinez, J. (2014) The mammalian tRNA ligase complex mediates splicing of XBP1 mRNA and controls antibody secretion in plasma cells. EMBO J. 33(24):2922-36
Klose, CS., Flach, M., Möhle, L., Rogell, L., Hoyler, T., Ebert, K., Fabiunke, C., Pfeifer, D., Sexl, V., Fonseca-Pereira, D., Domingues, RG., Veiga-Fernandes, H., Arnold, SJ., Busslinger, M., Dunay, IR., Tanriver, Y., Diefenbach, A. (2014) Differentiation of type 1 ILCs from a common progenitor to all helper-like innate lymphoid cell lineages. Cell. 157(2):340-56
Schwickert, TA., Tagoh, H., Gültekin, S., Dakic, A., Axelsson, E., Minnich, M., Ebert, A., Werner, B., Roth, M., Cimmino, L., Dickins, RA., Zuber, J., Jaritz, M., Busslinger, M. (2014) Stage-specific control of early B cell development by the transcription factor Ikaros. Nat Immunol. 15(3):283-93
Seillet, C., Huntington, ND., Gangatirkar, P., Axelsson, E., Minnich, M., Brady, HJ., Busslinger, M., Smyth, MJ., Belz, GT., Carotta, S. (2014) Differential requirement for Nfil3 during NK cell development. J Immunol. 192(6):2667-76
Ebert, A., Medvedovic, J., Tagoh, H., Schwickert, TA., Busslinger, M. (2013) Control of antigen receptor diversity through spatial regulation of V(D)J recombination. Cold Spring Harb Symp Quant Biol. 78:11-21
Esteghamat, F., Gillemans, N., Bilic, I., van den Akker, E., Cantù, I., van Gent, T., Klingmüller, U., van Lom, K., von Lindern, M., Grosveld, F., Bryn van Dijk, T., Busslinger, M., Philipsen, S. (2013) Erythropoiesis and globin switching in compound Klf1::Bcl11a mutant mice. Blood. 121(13):2553-62
Frelin, C., Herrington, R., Janmohamed, S., Barbara, M., Tran, G., Paige, CJ., Benveniste, P., Zuñiga-Pflücker, JC., Souabni, A., Busslinger, M., Iscove, NN. (2013) GATA-3 regulates the self-renewal of long-term hematopoietic stem cells. Nat Immunol. 14(10):1037-44
Hwang, SS., Kim, YU., Lee, S., Jang, SW., Kim, MK., Koh, BH., Lee, W., Kim, J., Souabni, A., Busslinger, M., Lee, GR. (2013) Transcription factor YY1 is essential for regulation of the Th2 cytokine locus and for Th2 cell differentiation. Proc Natl Acad Sci U S A. 110(1):276-81
Kollmann, K., Heller, G., Schneckenleithner, C., Warsch, W., Scheicher, R., Ott, RG., Schäfer, M., Fajmann, S., Schlederer, M., Schiefer, AI., Reichart, U., Mayerhofer, M., Hoeller, C., Zöchbauer-Müller, S., Kerjaschki, D., Bock, C., Kenner, L., Hoefler, G., Freissmuth, M., Green, AR., Moriggl, R., Busslinger, M., Malumbres, M., Sexl, V. (2013) A kinase-independent function of CDK6 links the cell cycle to tumor angiogenesis. Cancer Cell. 24(2):167-81
Masson, F., Minnich, M., Olshansky, M., Bilic, I., Mount, AM., Kallies, A., Speed, TP., Busslinger, M., Nutt, SL., Belz, GT. (2013) Id2-mediated inhibition of E2A represses memory CD8+ T cell differentiation. J Immunol. 190(9):4585-94
Medvedovic, J., Ebert, A., Tagoh, H., Tamir, IM., Schwickert, TA., Novatchkova, M., Sun, Q., Huis In 't Veld, PJ., Guo, C., Yoon, HS., Denizot, Y., Holwerda, SJ., de Laat, W., Cogné, M., Shi, Y., Alt, FW., Busslinger, M. (2013) Flexible long-range loops in the VH gene region of the Igh locus facilitate the generation of a diverse antibody repertoire. Immunity. 39(2):229-44
Ohtsuka, N., Badurek, S., Busslinger, M., Benes, FM., Minichiello, L., Rudolph, U. (2013) GABAergic neurons regulate lateral ventricular development via transcription factor Pax5. Genesis. 51(4):234-45
Demczuk, A., Gauthier, MG., Veras, I., Kosiyatrakul, S., Schildkraut, CL., Busslinger, M., Bechhoefer, J., Norio, P. (2012) Regulation of DNA replication within the immunoglobulin heavy-chain locus during B cell commitment. PLoS Biol. 10(7):e1001360
Hoyler, T., Klose, CS., Souabni, A., Turqueti-Neves, A., Pfeifer, D., Rawlins, EL., Voehringer, D., Busslinger, M., Diefenbach, A. (2012) The transcription factor GATA-3 controls cell fate and maintenance of type 2 innate lymphoid cells. Immunity. 37(4):634-48
Revilla-I-Domingo, R., Bilic, I., Vilagos, B., Tagoh, H., Ebert, A., Tamir, IM., Smeenk, L., Trupke, J., Sommer, A., Jaritz, M., Busslinger, M. (2012) The B-cell identity factor Pax5 regulates distinct transcriptional programmes in early and late B lymphopoiesis. EMBO J. 31(14):3130-46
Vilagos, B., Hoffmann, M., Souabni, A., Sun, Q., Werner, B., Medvedovic, J., Bilic, I., Minnich, M., Axelsson, E., Jaritz, M., Busslinger, M. (2012) Essential role of EBF1 in the generation and function of distinct mature B cell types. J Exp Med. 209(4):775-92
Cretney, E., Xin, A., Shi, W., Minnich, M., Masson, F., Miasari, M., Belz, GT., Smyth, GK., Busslinger, M., Nutt, SL., Kallies, A. (2011) The transcription factors Blimp-1 and IRF4 jointly control the differentiation and function of effector regulatory T cells. Nat Immunol. 12(4):304-11
Ebert, A., McManus, S., Tagoh, H., Medvedovic, J., Salvagiotto, G., Novatchkova, M., Tamir, I., Sommer, A., Jaritz, M., Busslinger, M. (2011) The distal V(H) gene cluster of the Igh locus contains distinct regulatory elements with Pax5 transcription factor-dependent activity in pro-B cells. Immunity. 34(2):175-87
Gimferrer, I., Hu, T., Simmons, A., Wang, C., Souabni, A., Busslinger, M., Bender, TP., Hernandez-Hoyos, G., Alberola-Ila, J. (2011) Regulation of GATA-3 expression during CD4 lineage differentiation. J Immunol. 186(7):3892-8
Guo, C., Yoon, HS., Franklin, A., Jain, S., Ebert, A., Cheng, HL., Hansen, E., Despo, O., Bossen, C., Vettermann, C., Bates, JG., Richards, N., Myers, D., Patel, H., Gallagher, M., Schlissel, MS., Murre, C., Busslinger, M., Giallourakis, CC., Alt, FW. (2011) CTCF-binding elements mediate control of V(D)J recombination. Nature. 477(7365):424-30
McManus, S., Ebert, A., Salvagiotto, G., Medvedovic, J., Sun, Q., Tamir, I., Jaritz, M., Tagoh, H., Busslinger, M. (2011) The transcription factor Pax5 regulates its target genes by recruiting chromatin-modifying proteins in committed B cells. EMBO J. 30(12):2388-404
Medvedovic, J., Ebert, A., Tagoh, H., Busslinger, M. (2011) Pax5: a master regulator of B cell development and leukemogenesis. Adv Immunol. 111:179-206
Qin, H., Suzuki, K., Nakata, M., Chikuma, S., Izumi, N., Huong, le T., Maruya, M., Fagarasan, S., Busslinger, M., Honjo, T., Nagaoka, H. (2011) Activation-induced cytidine deaminase expression in CD4+ T cells is associated with a unique IL-10-producing subset that increases with age. PLoS One. 6(12):e29141
Bouchard, M., de Caprona, D., Busslinger, M., Xu, P., Fritzsch, B. (2010) Pax2 and Pax8 cooperate in mouse inner ear morphogenesis and innervation. BMC Dev Biol. 10:89
Johnson, BA., Kahler, DJ., Baban, B., Chandler, PR., Kang, B., Shimoda, M., Koni, PA., Pihkala, J., Vilagos, B., Busslinger, M., Munn, DH., Mellor, AL. (2010) B-lymphoid cells with attributes of dendritic cells regulate T cells via indoleamine 2,3-dioxygenase. Proc Natl Acad Sci U S A. 107(23):10644-8
Majewski, IJ., Ritchie, ME., Phipson, B., Corbin, J., Pakusch, M., Ebert, A., Busslinger, M., Koseki, H., Hu, Y., Smyth, GK., Alexander, WS., Hilton, DJ., Blewitt, ME. (2010) Opposing roles of polycomb repressive complexes in hematopoietic stem and progenitor cells. Blood. 116(5):731-9
Malin, S., McManus, S., Busslinger, M. (2010) STAT5 in B cell development and leukemia. Curr Opin Immunol. 22(2):168-76
Malin, S., McManus, S., Cobaleda, C., Novatchkova, M., Delogu, A., Bouillet, P., Strasser, A., Busslinger, M. (2010) Role of STAT5 in controlling cell survival and immunoglobulin gene recombination during pro-B cell development. Nat Immunol. 11(2):171-9
Vikstrom, I., Carotta, S., Lüthje, K., Peperzak, V., Jost, PJ., Glaser, S., Busslinger, M., Bouillet, P., Strasser, A., Nutt, SL., Tarlinton, DM. (2010) Mcl-1 is essential for germinal center formation and B cell memory. Science. 330(6007):1095-9
Agrelo, R., Souabni, A., Novatchkova, M., Haslinger, C., Leeb, M., Komnenovic, V., Kishimoto, H., Gresh, L., Kohwi-Shigematsu, T., Kenner, L., Wutz, A. (2009) SATB1 defines the developmental context for gene silencing by Xist in lymphoma and embryonic cells. Dev Cell. 16(4):507-16
Decker, T., Pasca di Magliano, M., McManus, S., Sun, Q., Bonifer, C., Tagoh, H., Busslinger, M. (2009) Stepwise activation of enhancer and promoter regions of the B cell commitment gene Pax5 in early lymphopoiesis. Immunity. 30(4):508-20
Hewitt, SL., Yin, B., Ji, Y., Chaumeil, J., Marszalek, K., Tenthorey, J., Salvagiotto, G., Steinel, N., Ramsey, LB., Ghysdael, J., Farrar, MA., Sleckman, BP., Schatz, DG., Busslinger, M., Bassing, CH., Skok, JA. (2009) RAG-1 and ATM coordinate monoallelic recombination and nuclear positioning of immunoglobulin loci. Nat Immunol. 10(6):655-64
Cobaleda, C., Busslinger, M. (2008) Developmental plasticity of lymphocytes. Curr Opin Immunol. 20(2):139-48
Graf, T., Busslinger, M. (2008) B young again. Immunity. 28(5):606-8
Guo, F., Tänzer, S., Busslinger, M., Weih, F. (2008) Lack of nuclear factor-kappa B2/p100 causes a RelB-dependent block in early B lymphopoiesis. Blood. 112(3):551-9
Kwon, K., Hutter, C., Sun, Q., Bilic, I., Cobaleda, C., Malin, S., Busslinger, M. (2008) Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development. Immunity. 28(6):751-62
Schotta, G., Sengupta, R., Kubicek, S., Malin, S., Kauer, M., Callén, E., Celeste, A., Pagani, M., Opravil, S., De La Rosa-Velazquez, IA., Espejo, A., Bedford, MT., Nussenzweig, A., Busslinger, M., Jenuwein, T. (2008) A chromatin-wide transition to H4K20 monomethylation impairs genome integrity and programmed DNA rearrangements in the mouse. Genes Dev. 22(15):2048-61
Amsen, D., Antov, A., Jankovic, D., Sher, A., Radtke, F., Souabni, A., Busslinger, M., McCright, B., Gridley, T., Flavell, RA. (2007) Direct regulation of Gata3 expression determines the T helper differentiation potential of Notch. Immunity. 27(1):89-99
Bruce, SJ., Rea, RW., Steptoe, AL., Busslinger, M., Bertram, JF., Perkins, AC. (2007) In vitro differentiation of murine embryonic stem cells toward a renal lineage. Differentiation. 75(5):337-49
Cobaleda, C., Jochum, W., Busslinger, M. (2007) Conversion of mature B cells into T cells by dedifferentiation to uncommitted progenitors. Nature. 449(7161):473-7
Cobaleda, C., Schebesta, A., Delogu, A., Busslinger, M. (2007) Pax5: the guardian of B cell identity and function. Nat Immunol. 8(5):463-70
Martinez, J., Busslinger, M. (2007) Life beyond cleavage: the case of Ago2 and hematopoiesis. Genes Dev. 21(16):1983-8
Roessler, S., Györy, I., Imhof, S., Spivakov, M., Williams, RR., Busslinger, M., Fisher, AG., Grosschedl, R. (2007) Distinct promoters mediate the regulation of Ebf1 gene expression by interleukin-7 and Pax5. Mol Cell Biol. 27(2):579-94
Schebesta, A., McManus, S., Salvagiotto, G., Delogu, A., Busslinger, GA., Busslinger, M. (2007) Transcription factor Pax5 activates the chromatin of key genes involved in B cell signaling, adhesion, migration, and immune function. Immunity. 27(1):49-63
Skok, JA., Gisler, R., Novatchkova, M., Farmer, D., de Laat, W., Busslinger, M. (2007) Reversible contraction by looping of the Tcra and Tcrb loci in rearranging thymocytes. Nat Immunol. 8(4):378-87
Busslinger, M., Tarakhovsky, A. (2006) Epigenetic control of lymphopoiesis. In Epigenetics (Allis, Jenuwein, Reinberg, eds.); Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.. :397-414
Delogu, A., Schebesta, A., Sun, Q., Aschenbrenner, K., Perlot, T., Busslinger, M. (2006) Gene repression by Pax5 in B cells is essential for blood cell homeostasis and is reversed in plasma cells. Immunity. 24(3):269-81
Grote, D., Souabni, A., Busslinger, M., Bouchard, M. (2006) Pax 2/8-regulated Gata 3 expression is necessary for morphogenesis and guidance of the nephric duct in the developing kidney. Development. 133(1):53-61
Savarese, F., Flahndorfer, K., Jaenisch, R., Busslinger, M., Wutz, A. (2006) Hematopoietic precursor cells transiently reestablish permissiveness for X inactivation. Mol Cell Biol. 26(19):7167-77
Tagoh, H., Ingram, R., Wilson, N., Salvagiotto, G., Warren, AJ., Clarke, D., Busslinger, M., Bonifer, C. (2006) The mechanism of repression of the myeloid-specific c-fms gene by Pax5 during B lineage restriction. EMBO J. 25(5):1070-80
Weisheit, G., Gliem, M., Endl, E., Pfeffer, PL., Busslinger, M., Schilling, K. (2006) Postnatal development of the murine cerebellar cortex: formation and early dispersal of basket, stellate and Golgi neurons. Eur J Neurosci. 24(2):466-78
Ardouin, L., Rolink, AG., Mura, AM., Gommeaux, J., Melchers, F., Busslinger, M., Malissen, M., Malissen, B. (2005) Rapid in vivo analysis of mutant forms of the LAT adaptor using Pax5-Lat double-deficient pro-B cells. Eur J Immunol. 35(3):977-86
Bouchard, M., Grote, D., Craven, SE., Sun, Q., Steinlein, P., Busslinger, M. (2005) Identification of Pax2-regulated genes by expression profiling of the mid-hindbrain organizer region. Development. 132(11):2633-43
Pelayo, R., Hirose, J., Huang, J., Garrett, KP., Delogu, A., Busslinger, M., Kincade, PW. (2005) Derivation of 2 categories of plasmacytoid dendritic cells in murine bone marrow. Blood. 105(11):4407-15
Roldán, E., Fuxa, M., Chong, W., Martinez, D., Novatchkova, M., Busslinger, M., Skok, JA. (2005) Locus 'decontraction' and centromeric recruitment contribute to allelic exclusion of the immunoglobulin heavy-chain gene. Nat Immunol. 6(1):31-41
Bouchard, M., Souabni, A., Busslinger, M. (2004) Tissue-specific expression of cre recombinase from the Pax8 locus. Genesis. 38(3):105-9
Busslinger, M. (2004) Transcriptional control of early B cell development. Annu Rev Immunol. 22:55-79
Cheng, L., Arata, A., Mizuguchi, R., Qian, Y., Karunaratne, A., Gray, PA., Arata, S., Shirasawa, S., Bouchard, M., Luo, P., Chen, CL., Busslinger, M., Goulding, M., Onimaru, H., Ma, Q. (2004) Tlx3 and Tlx1 are post-mitotic selector genes determining glutamatergic over GABAergic cell fates. Nat Neurosci. 7(5):510-7
Fuxa, M., Skok, J., Souabni, A., Salvagiotto, G., Roldan, E., Busslinger, M. (2004) Pax5 induces V-to-DJ rearrangements and locus contraction of the immunoglobulin heavy-chain gene. Genes Dev. 18(4):411-22
Höflinger, S., Kesavan, K., Fuxa, M., Hutter, C., Heavey, B., Radtke, F., Busslinger, M. (2004) Analysis of Notch1 function by in vitro T cell differentiation of Pax5 mutant lymphoid progenitors. J Immunol. 173(6):3935-44
Linderson, Y., Eberhard, D., Malin, S., Johansson, A., Busslinger, M., Pettersson, S. (2004) Corecruitment of the Grg4 repressor by PU.1 is critical for Pax5-mediated repression of B-cell-specific genes. EMBO Rep. 5(3):291-6
Tagoh, H., Schebesta, A., Lefevre, P., Wilson, N., Hume, D., Busslinger, M., Bonifer, C. (2004) Epigenetic silencing of the c-fms locus during B-lymphopoiesis occurs in discrete steps and is reversible. EMBO J. 23(21):4275-85
Heavey, B., Charalambous, C., Cobaleda, C., Busslinger, M. (2003) Myeloid lineage switch of Pax5 mutant but not wild-type B cell progenitors by C/EBPalpha and GATA factors. EMBO J. 22(15):3887-97
Bouchard, M., Souabni, A., Mandler, M., Neubüser, A., Busslinger, M. (2002) Nephric lineage specification by Pax2 and Pax8. Genes Dev. 16(22):2958-70
Mikkola, I., Heavey, B., Horcher, M., Busslinger, M. (2002) Reversion of B cell commitment upon loss of Pax5 expression. Science. 297(5578):110-3
Pfeffer, PL., Payer, B., Reim, G., di Magliano, MP., Busslinger, M. (2002) The activation and maintenance of Pax2 expression at the mid-hindbrain boundary is controlled by separate enhancers. Development. 129(2):307-18
Schebesta, M., Heavey, B., Busslinger, M. (2002) Transcriptional control of B-cell development. Curr Opin Immunol. 14(2):216-23
Schebesta, M., Pfeffer, PL., Busslinger, M. (2002) Control of pre-BCR signaling by Pax5-dependent activation of the BLNK gene. Immunity. 17(4):473-85
Souabni, A., Cobaleda, C., Schebesta, M., Busslinger, M. (2002) Pax5 promotes B lymphopoiesis and blocks T cell development by repressing Notch1. Immunity. 17(6):781-93
Ellis, T., Gambardella, L., Horcher, M., Tschanz, S., Capol, J., Bertram, P., Jochum, W., Barrandon, Y., Busslinger, M. (2001) The transcriptional repressor CDP (Cutl1) is essential for epithelial cell differentiation of the lung and the hair follicle. Genes Dev. 15(17):2307-19
Horcher, M., Eberhard, D., Busslinger, M. (2001) The role of PAX5 (BSAP) in early and late B-cell development. Transcription Factors: Normal and Malignant Development of Blood Cells (Ravid, K., Licht, Jd., eds.). :217-228
Horcher, M., Souabni, A., Busslinger, M. (2001) Pax5/BSAP maintains the identity of B cells in late B lymphopoiesis. Immunity. 14(6):779-90
Nutt, SL., Eberhard, D., Horcher, M., Rolink, AG., Busslinger, M. (2001) Pax5 determines the identity of B cells from the beginning to the end of B-lymphopoiesis. Int Rev Immunol. 20(1):65-82
Ye, W., Bouchard, M., Stone, D., Liu, X., Vella, F., Lee, J., Nakamura, H., Ang, SL., Busslinger, M., Rosenthal, A. (2001) Distinct regulators control the expression of the mid-hindbrain organizer signal FGF8. Nat Neurosci. 4(12):1175-81
Bouchard, M., Pfeffer, P., Busslinger, M. (2000) Functional equivalence of the transcription factors Pax2 and Pax5 in mouse development. Development. 127(17):3703-13
Busslinger, M., Nutt, SL., Rolink, AG. (2000) Lineage commitment in lymphopoiesis. Curr Opin Immunol. 12(2):151-8
Eberhard, D., Jiménez, G., Heavey, B., Busslinger, M. (2000) Transcriptional repression by Pax5 (BSAP) through interaction with corepressors of the Groucho family. EMBO J. 19(10):2292-303
Revy, P., Busslinger, M., Tashiro, K., Arenzana, F., Pillet, P., Fischer, A., Durandy, A. (2000) A syndrome involving intrauterine growth retardation, microcephaly, cerebellar hypoplasia, B lymphocyte deficiency, and progressive pancytopenia. Pediatrics. 105(3):E39
Rolink, AG., Schaniel, C., Busslinger, M., Nutt, SL., Melchers, F. (2000) Fidelity and infidelity in commitment to B-lymphocyte lineage development. Immunol Rev. 175:104-11
Czerny, T., Halder, G., Kloter, U., Souabni, A., Gehring, WJ., Busslinger, M. (1999) twin of eyeless, a second Pax-6 gene of Drosophila, acts upstream of eyeless in the control of eye development. Mol Cell. 3(3):297-307
Eberhard, D., Busslinger, M. (1999) The partial homeodomain of the transcription factor Pax-5 (BSAP) is an interaction motif for the retinoblastoma and TATA-binding proteins. Cancer Res. 59(7 Suppl):1716s-1724s; discussion 1724s-1725s
Nutt, SL., Heavey, B., Rolink, AG., Busslinger, M. (1999) Commitment to the B-lymphoid lineage depends on the transcription factor Pax5. Nature. 401(6753):556-62
Nutt, SL., Rolink, AG., Busslinger, M. (1999) The molecular basis of B-cell lineage commitment. Cold Spring Harb Symp Quant Biol. 64:51-9
Nutt, SL., Vambrie, S., Steinlein, P., Kozmik, Z., Rolink, A., Weith, A., Busslinger, M. (1999) Independent regulation of the two Pax5 alleles during B-cell development. Nat Genet. 21(4):390-5
Rolink, A., Nutt, S., Busslinger, M., ten Boekel, E., Seidl, T., Andersson, J., Melchers, F. (1999) Differentiation, dedifferentiation, and redifferentiation of B-lineage lymphocytes: roles of the surrogate light chain and the Pax5 gene. Cold Spring Harb Symp Quant Biol. 64:21-5
Rolink, AG., Nutt, SL., Melchers, F., Busslinger, M. (1999) Long-term in vivo reconstitution of T-cell development by Pax5-deficient B-cell progenitors. Nature. 401(6753):603-6
Schwarz, M., Alvarez-Bolado, G., Dressler, G., Urbánek, P., Busslinger, M., Gruss, P. (1999) Pax2/5 and Pax6 subdivide the early neural tube into three domains. Mech Dev. 82(1-2):29-39
Busslinger, M., Nutt, SL. (1998) Role of the transcription factor BSAP [Pax-5] in B-cell development. Molecular Biology of B-Cell and T-Cell Development (eds. Monroe, JG., Rothenberg, EV.). :83-110
Macchia, PE., Lapi, P., Krude, H., Pirro, MT., Missero, C., Chiovato, L., Souabni, A., Baserga, M., Tassi, V., Pinchera, A., Fenzi, G., Grüters, A., Busslinger, M., Di Lauro, R. (1998) PAX8 mutations associated with congenital hypothyroidism caused by thyroid dysgenesis. Nat Genet. 19(1):83-6
Morrison, AM., Jäger, U., Chott, A., Schebesta, M., Haas, OA., Busslinger, M. (1998) Deregulated PAX-5 transcription from a translocated IgH promoter in marginal zone lymphoma. Blood. 92(10):3865-78
Morrison, AM., Nutt, SL., Thévenin, C., Rolink, A., Busslinger, M. (1998) Loss- and gain-of-function mutations reveal an important role of BSAP (Pax-5) at the start and end of B cell differentiation. Semin Immunol. 10(2):133-42
Nutt, SL., Morrison, AM., Dörfler, P., Rolink, A., Busslinger, M. (1998) Identification of BSAP (Pax-5) target genes in early B-cell development by loss- and gain-of-function experiments. EMBO J. 17(8):2319-33
Pfeffer, PL., Gerster, T., Lun, K., Brand, M., Busslinger, M. (1998) Characterization of three novel members of the zebrafish Pax2/5/8 family: dependency of Pax5 and Pax8 expression on the Pax2.1 (noi) function. Development. 125(16):3063-74
Thévenin, C., Nutt, SL., Busslinger, M. (1998) Early function of Pax5 (BSAP) before the pre-B cell receptor stage of B lymphopoiesis. J Exp Med. 188(4):735-44
Brandt, WF., Schwager, SU., Rodrigues, JA., Busslinger, M. (1997) Isolation and amino acid sequence analysis reveal an ancient evolutionary origin of the cleavage stage (CS) histones of the sea urchin. Eur J Biochem. 247(3):784-91
Chlichlia, K., Busslinger, M., Peter, ME., Walczak, H., Krammer, PH., Schirrmacher, V., Khazaie, K. (1997) ICE-proteases mediate HTLV-I Tax-induced apoptotic T-cell death. Oncogene. 14(19):2265-72
De Monte, L., Thienes, CP., Monticelli, S., Busslinger, M., Gould, HJ., Vercelli, D. (1997) Regulation of human epsilon germline transcription: role of B-cell-specific activator protein. Int Arch Allergy Immunol. 113(1-3):35-8
Nutt, SL., Thévenin, C., Busslinger, M. (1997) Essential functions of Pax-5 (BSAP) in pro-B cell development. Immunobiology. 198(1-3):227-35
Schwarz, M., Alvarez-Bolado, G., Urbánek, P., Busslinger, M., Gruss, P. (1997) Conserved biological function between Pax-2 and Pax-5 in midbrain and cerebellum development: evidence from targeted mutations. Proc Natl Acad Sci U S A. 94(26):14518-23
Thienes, CP., De Monte, L., Monticelli, S., Busslinger, M., Gould, HJ., Vercelli, D. (1997) The transcription factor B cell-specific activator protein (BSAP) enhances both IL-4- and CD40-mediated activation of the human epsilon germline promoter. J Immunol. 158(12):5874-82
Urbánek, P., Fetka, I., Meisler, MH., Busslinger, M. (1997) Cooperation of Pax2 and Pax5 in midbrain and cerebellum development. Proc Natl Acad Sci U S A. 94(11):5703-8
Busslinger, M., Klix, N., Pfeffer, P., Graninger, PG., Kozmik, Z. (1996) Deregulation of PAX-5 by translocation of the Emu enhancer of the IgH locus adjacent to two alternative PAX-5 promoters in a diffuse large-cell lymphoma. Proc Natl Acad Sci U S A. 93(12):6129-34
Fialka, I., Schwarz, H., Reichmann, E., Oft, M., Busslinger, M., Beug, H. (1996) The estrogen-dependent c-JunER protein causes a reversible loss of mammary epithelial cell polarity involving a destabilization of adherens junctions. J Cell Biol. 132(6):1115-32
Bergers, G., Graninger, P., Braselmann, S., Wrighton, C., Busslinger, M. (1995) Transcriptional activation of the fra-1 gene by AP-1 is mediated by regulatory sequences in the first intron. Mol Cell Biol. 15(7):3748-58
Busslinger, M., Urbánek, P. (1995) The role of BSAP (Pax-5) in B-cell development. Curr Opin Genet Dev. 5(5):595-601
Chlichlia, K., Moldenhauer, G., Daniel, PT., Busslinger, M., Gazzolo, L., Schirrmacher, V., Khazaie, K. (1995) Immediate effects of reversible HTLV-1 tax function: T-cell activation and apoptosis. Oncogene. 10(2):269-77
Kozmik, Z., Sure, U., Rüedi, D., Busslinger, M., Aguzzi, A. (1995) Deregulated expression of PAX5 in medulloblastoma. Proc Natl Acad Sci U S A. 92(12):5709-13
Stapleton, P., Kozmik, Z., Weith, A., Busslinger, M. (1995) The gene coding for the B cell surface protein CD19 is localized on human chromosome 16p11. Hum Genet. 95(2):223-5
Vorechovský, I., Koskinen, S., Paganelli, R., Smith, CI., Busslinger, M., Hammarström, L. (1995) The PAX5 gene: a linkage and mutation analysis in candidate human primary immunodeficiencies. Immunogenetics. 42(2):149-52
Busslinger, M., Bergers, G. (1994) Identification of AP-1-regulated genes. The Fos and Jun families of transcription factors (eds. Angel, PE., Herrlich, PA.). :133-50
Liao, F., Birshtein, BK., Busslinger, M., Rothman, P. (1994) The transcription factor BSAP (NF-HB) is essential for immunoglobulin germ-line epsilon transcription. J Immunol. 152(6):2904-11
Schäfer, BW., Czerny, T., Bernasconi, M., Genini, M., Busslinger, M. (1994) Molecular cloning and characterization of a human PAX-7 cDNA expressed in normal and neoplastic myocytes. Nucleic Acids Res. 22(22):4574-82
Urbánek, P., Wang, ZQ., Fetka, I., Wagner, EF., Busslinger, M. (1994) Complete block of early B cell differentiation and altered patterning of the posterior midbrain in mice lacking Pax5/BSAP. Cell. 79(5):901-12
Vorechovský, I., Kozmík, Z., Hammarström, L., Smith, CI., Busslinger, M. (1994) An intragenic TaqI RFLP at the PAX5 locus. Hum Mol Genet. 3(4):681
Vorechovský, I., Kurzbauer, R., Kozmík, Z., Hammarström, L., Smith, CI., Busslinger, M. (1994) SSCP/SacI polymorphism in the PAX5 gene. Hum Mol Genet. 3(5):839
Braselmann, S., Graninger, P., Busslinger, M. (1993) A selective transcriptional induction system for mammalian cells based on Gal4-estrogen receptor fusion proteins. Proc Natl Acad Sci U S A. 90(5):1657-61
Czerny, T., Schaffner, G., Busslinger, M. (1993) DNA sequence recognition by Pax proteins: bipartite structure of the paired domain and its binding site. Genes Dev. 7(10):2048-61
Kozmik, Z., Kurzbauer, R., Dörfler, P., Busslinger, M. (1993) Alternative splicing of Pax-8 gene transcripts is developmentally regulated and generates isoforms with different transactivation properties. Mol Cell Biol. 13(10):6024-35
Stapleton, P., Weith, A., Urbánek, P., Kozmik, Z., Busslinger, M. (1993) Chromosomal localization of seven PAX genes and cloning of a novel family member, PAX-9. Nat Genet. 3(4):292-8
Adams, B., Dörfler, P., Aguzzi, A., Kozmik, Z., Urbánek, P., Maurer-Fogy, I., Busslinger, M. (1992) Pax-5 encodes the transcription factor BSAP and is expressed in B lymphocytes, the developing CNS, and adult testis. Genes Dev. 6(9):1589-607
Braselmann, S., Bergers, G., Wrighton, C., Graninger, P., Superti-Furga, G., Busslinger, M. (1992) Identification of Fos target genes by the use of selective induction systems. J Cell Sci Suppl. 16:97-109
Kozmik, Z., Wang, S., Dörfler, P., Adams, B., Busslinger, M. (1992) The promoter of the CD19 gene is a target for the B-cell-specific transcription factor BSAP. Mol Cell Biol. 12(6):2662-72
Reichmann, E., Schwarz, H., Deiner, EM., Leitner, I., Eilers, M., Berger, J., Busslinger, M., Beug, H. (1992) Activation of an inducible c-FosER fusion protein causes loss of epithelial polarity and triggers epithelial-fibroblastoid cell conversion. Cell. 71(7):1103-16
Superti-Furga, G., Bergers, G., Picard, D., Busslinger, M. (1991) Hormone-dependent transcriptional regulation and cellular transformation by Fos-steroid receptor fusion proteins. Proc Natl Acad Sci U S A. 88(12):5114-8
Barberis, A., Widenhorn, K., Vitelli, L., Busslinger, M. (1990) A novel B-cell lineage-specific transcription factor present at early but not late stages of differentiation. Genes Dev. 4(5):849-59
Barberis, A., Superti-Furga, G., Busslinger, M. (1989) The testis-specific octamer-binding protein of the sea urchin has a molecular weight of 85 kDa. Nucleic Acids Res. 17(17):7114
Barberis, A., Superti-Furga, G., Vitelli, L., Kemler, I., Busslinger, M. (1989) Developmental and tissue-specific regulation of a novel transcription factor of the sea urchin. Genes Dev. 3(5):663-75
Birnstiel, ML., Busslinger, M. (1989) Dangerous liaisons: spermatozoa as natural vectors for foreign DNA? Cell. 57(5):701-2
Birnstiel, ML., Busslinger, M. (1989) Further liaisons with sperm. Science. 245(4915):243-4
Superti-Furga, G., Barberis, A., Schreiber, E., Busslinger, M. (1989) The protein CDP, but not CP1, footprints on the CCAAT region of the gamma-globin gene in unfractionated B-cell extracts. Biochim Biophys Acta. 1007(2):237-42
Cosson, J., Kemler, I., Tanno, S., Busslinger, M. (1988) Promoter of a somatic histone H2B gene of the sea urchin. Nucleic Acids Res. 16(9):4175
Superti-Furga, G., Barberis, A., Schaffner, G., Busslinger, M. (1988) The -117 mutation in Greek HPFH affects the binding of three nuclear factors to the CCAAT region of the gamma-globin gene. EMBO J. 7(10):3099-107
Vitelli, L., Kemler, I., Lauber, B., Birnstiel, ML., Busslinger, M. (1988) Developmental regulation of micro-injected histone genes in sea urchin embryos. Dev Biol. 127(1):54-63
Birnstiel, ML., Busslinger, M., Strub, K. (1985) Transcription termination and 3' processing: the end is in site! Cell. 41(2):349-59
Busslinger, M., Schümperli, D., Birnstiel, ML. (1985) Regulation of histone gene expression. Cold Spring Harb Symp Quant Biol. 50:665-70
Busslinger, M., deBoer, E., Wright, S., Grosveld, FG., Flavell, RA. (1983) The sequence GGCmCGG is resistant to MspI cleavage. Nucleic Acids Res. 11(11):3559-69
Busslinger, M., Flavell, RA. (1983) DNA methylation and the regulation of globin gene expression. Prog Clin Biol Res. 134:193-203
Busslinger, M., Hurst, J., Flavell, RA. (1983) DNA methylation and the regulation of globin gene expression. Cell. 34(1):197-206
Flavell, RA., Grosveld, F., Busslinger, M., de Boer, E., Kioussis, D., Mellor, AL., Golden, L., Weiss, E., Hurst, J., Bud, H., Bullman, H., Simpson, E., James, R., Townsend, AR., Taylor, PM., Schmidt, W., Ferluga, J., Leben, L., Santamaria, M., Atfield, G., Festenstein, H. (1983) Structure and expression of the human globin genes and murine histocompatibility antigen genes. Cold Spring Harb Symp Quant Biol. 47 Pt 2:1067-78
Flavell, RA., Bud, H., Bullman, H., Busslinger, M., deBoer, E., deKleine, A., Golden, L., Groffen, J., Grosveld, FG., Mellor, AL., Moschonas, N., Weiss, E. (1982) The structure and expression of mammalian gene clusters. Prog Clin Biol Res. 103 Pt A:37-55
Flavell, RA., Moschonas, NK., deBoer, E., Grosveld, GC., Busslinger, M., Dahl, HH., Grosveld, FG. (1982) Phenotypic analysis of globin gene expression: the thalassaemias. Prog Clin Biol Res. 85 Pt A:25-39
Grosveld, F., Busslinger, M., Grosveld, G., Groffen, J., DeKleine, A., Flavell, RA. (1982) The structure and expression of the haemoglobin genes. Adv Exp Med Biol. 158:65-80
Busslinger, M., Moschonas, N., Flavell, RA. (1981) Beta + thalassemia: aberrant splicing results from a single point mutation in an intron. Cell. 27(2 Pt 1):289-98
Busslinger, M., Portmann, R., Irminger, JC., Birnstiel, ML. (1980) Ubiquitous and gene-specific regulatory 5' sequences in a sea urchin histone DNA clone coding for histone protein variants. Nucleic Acids Res. 8(5):957-77
Busslinger, M., Portmann, R., Birnsteil, ML. (1979) A regulatory sequence near the 3' end of sea urchin histone genes. Nucleic Acids Res. 6(9):2997-3008
Birnstiel, ML., Kressmann, A., Schaffner, W., Portmann, R., Busslinger, M. (1978) Aspects of the regulation of histone genes. Philos Trans R Soc Lond B Biol Sci. 283(997):319-24
Cherry, RJ., Bürkli, A., Busslinger, M., Schneider, G., Parish, GR. (1976) Rotational diffusion of band 3 proteins in the human erythrocyte membrane. Nature. 263(5576):389-93