Tim Clausen

BacPROTAC-induced protein degradation as a new antibiotic concept.

PARG regulates the proteasomal degradation of TARG1.

Structural basis for substrate recruitment and catalytic ubiquitin transfer by the E2/E3 hybrid enzyme UBE2O.

BORC assemblies integrate BLOC-1 subunits to diversify endosomal trafficking functions.

Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity.

Cross-species interactome analysis uncovers a conserved selective autophagy mechanism for protein quality control in plants.

Architecture of the UBR4 complex, a giant E4 ligase central to eukaryotic protein quality control.

A split-site E3 ligase mechanism enables ZNFX1 to ubiquitinate and cluster single-stranded RNA into ubiquitin-coated nucleoprotein particles.

ATP functions as a pathogen-associated molecular pattern to activate the E3 ubiquitin ligase RNF213.

TRIM52 maintains cellular fitness and is under tight proteolytic control by multiple giant E3 ligases.

Protein waste turned into antibiotics as a defence strategy of human cells.

Convergence of orphan quality control pathways at a ubiquitin chain-elongating ligase.

UNC-45 assisted myosin folding depends on a conserved FX3HY motif implicated in Freeman Sheldon Syndrome.

Rational correction of pathogenic conformational defects in HTRA1.

TRIM52 is a primate-specific player in the DNA repair process under tight proteolytic control by a triad of giant E3 ligases

Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity

Multiple ubiquitin ligases protect human genome integrity by targeting cancer-associated APOBEC3 deaminases for degradation

Homo-BacPROTAC-induced degradation of ClpC1 as a strategy against drug-resistant mycobacteria.

Clp-targeting BacPROTACs impair mycobacterial proteostasis and survival.

HUWE1 controls tristetraprolin proteasomal degradation by regulating its phosphorylation.

Structural basis for regulation of apoptosis and autophagy by the BIRC6/SMAC complex.

Shuffled ATG8 interacting motifs form an ancestral bridge between UFMylation and autophagy.

BacPROTACs mediate targeted protein degradation in bacteria.

Moyamoya disease: diagnosis and interventions.

Targeted protein degradation: from small molecules to complex organelles-a Keystone Symposia report.

Potent Bactericidal Antimycobacterials Targeting the Chaperone ClpC1 Based on the Depsipeptide Natural Products Ecumicin and Ohmyungsamycin A.

An allosteric HTRA1-calpain 2 complex with restricted activation profile.

The linear ubiquitin chain assembly complex (LUBAC) generates heterotypic ubiquitin chains.

McsB forms a gated kinase chamber to mark aberrant bacterial proteins for degradation.

HUWE1 employs a giant substrate-binding ring to feed and regulate its HECT E3 domain.

The oxidoreductase PYROXD1 uses NAD(P)+ as an antioxidant to sustain tRNA ligase activity in pre-tRNA splicing and unfolded protein response.

Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq.

E3 ubiquitin ligase RNF213 employs a non-canonical zinc finger active site and is allosterically regulated by ATP

A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress.

Moyamoya disease factor RNF213 is a giant E3 ligase with a dynein-like core and a distinct ubiquitin-transfer mechanism.

Native Mass Spectrometry Can Effectively Predict PROTAC Efficacy.

Shaping Striated Muscles with Ubiquitin Proteasome System in Health and Disease.

Activation by substoichiometric inhibition.

Molecular features of the UNC-45 chaperone critical for binding and folding muscle myosin.

Structure of McsB, a protein kinase for regulated arginine phosphorylation.

Chemical Biology Interrogates Protein Arginine Phosphorylation.

The crystal structure of Deg9 reveals a novel octameric-type HtrA protease.

UFD-2 is an adaptor-assisted E3 ligase targeting unfolded proteins.

Structural mechanism for the recognition and ubiquitination of a single nucleosome residue by Rad6-Bre1.

Chasing Phosphoarginine Proteins: Development of a Selective Enrichment Method Using a Phosphatase Trap.

Structural basis for the disaggregase activity and regulation of Hsp104.

Arginine phosphorylation marks proteins for degradation by a Clp protease.

Determinants of amyloid fibril degradation by the PDZ protease HTRA1.

Versatile in vitro system to study translocation and functional integration of bacterial outer membrane proteins.

The Nuclear Pore-Associated TREX-2 Complex Employs Mediator to Regulate Gene Expression.

Myosin chaperones.

Quantitative phosphoproteomics reveals the role of protein arginine phosphorylation in the bacterial stress response.

RNA specificity and regulation of catalysis in the eukaryotic polynucleotide kinase Clp1.

Human CLP1 mutations alter tRNA biogenesis, affecting both peripheral and central nervous system function.

Structural basis for recognizing phosphoarginine and evolving residue-specific protein phosphatases in gram-positive bacteria.

CtpB assembles a gated protease tunnel regulating cell-cell signaling during spore formation in Bacillus subtilis.

Mutations in the β-tubulin gene TUBB5 cause microcephaly with structural brain abnormalities.

A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors.

The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans.

Stress-induced GSK3 regulates the redox stress response by phosphorylating glucose-6-phosphate dehydrogenase in Arabidopsis.

Human high temperature requirement serine protease A1 (HTRA1) degrades tau protein aggregates.

Newly folded substrates inside the molecular cage of the HtrA chaperone DegQ.

Protein quality control in the bacterial periplasm.

Molecular adaptation of the DegQ protease to exert protein quality control in the bacterial cell envelope.

Structural adaptation of the plant protease Deg1 to repair photosystem II during light exposure.

Substrate-induced remodeling of the active site regulates human HTRA1 activity.

Augmenting β-augmentation: structural basis of how BamB binds BamA and may support folding of outer membrane proteins.

HTRA proteases: regulated proteolysis in protein quality control.

Molecular transformers in the cell: lessons learned from the DegP protease-chaperone.

HtrA proteases have a conserved activation mechanism that can be triggered by distinct molecular cues.

Conversion of a regulatory into a degradative protease.

Stoichiometry determination of the MP1-p14 complex using a novel and cost-efficient method to produce an equimolar mixture of standard peptides.

Structure, function and regulation of the conserved serine proteases DegP and DegS of Escherichia coli.

Peptidic small molecule activators of the stress sensor DegS.

Structural basis of substrate specificity of plant 12-oxophytodienoate reductases.

McsB is a protein arginine kinase that phosphorylates and inhibits the heat-shock regulator CtsR.

Selectivity profiling of DegP substrates and inhibitors.

Structural basis for the regulated protease and chaperone function of DegP.

Interplay of PDZ and protease domain of DegP ensures efficient elimination of misfolded proteins.

Allosteric activation of HtrA protease DegP by stress signals during bacterial protein quality control.

Regulation of the sigmaE stress response by DegS: how the PDZ domain keeps the protease inactive in the resting state and allows integration of different OMP-derived stress signals upon folding stress.

Crystal structure of 12-oxophytodienoate reductase 3 from tomato: self-inhibition by dimerization.

The role of human HtrA1 in arthritic disease.

TIS7 regulation of the beta-catenin/Tcf-4 target gene osteopontin (OPN) is histone deacetylase-dependent.

The 1.3 A crystal structure of the flavoprotein YqjM reveals a novel class of Old Yellow Enzymes.

Molecular machines for protein degradation.

Implications of the serine protease HtrA1 in amyloid precursor protein processing.

Proteolysis as a regulatory mechanism.

Structural and functional impairment of an Old Yellow Enzyme homologue upon affinity tag incorporation.

Crystal structure of the p14/MP1 scaffolding complex: how a twin couple attaches mitogen-activated protein kinase signaling to late endosomes.

Molecular shredders: how proteasomes fulfill their role.

Crystal structure of the DegS stress sensor: How a PDZ domain recognizes misfolded protein and activates a protease.

Muscle regeneration and myogenic differentiation defects in mice lacking TIS7.

Snapshots of the cystine lyase C-DES during catalysis. Studies in solution and in the crystalline state.

Enzyme-ligand complexes of pyridoxine 5'-phosphate synthase: implications for substrate binding and catalysis.

The HtrA family of proteases: implications for protein composition and cell fate.

Spectroscopic and kinetic analyses reveal the pyridoxal 5'-phosphate binding mode and the catalytic features of Treponema denticola cystalysin.

Crystal structure of DegP (HtrA) reveals a new protease-chaperone machine.

Structure and function of threonine synthase from yeast.

Crystal structure of transcription factor MalT domain III: a novel helix repeat fold implicated in regulated oligomerization.

X-ray structure of 12-oxophytodienoate reductase 1 provides structural insight into substrate binding and specificity within the family of OYE.

The three-dimensional structure of cystathionine beta-lyase from Arabidopsis and its substrate specificity.

Crystal structures of cystathionine gamma-synthase inhibitor complexes rationalize the increased affinity of a novel inhibitor.

Structural basis for the function of pyridoxine 5'-phosphate synthase.

Crystallization and preliminary X-ray crystallographic analysis of PdxJ, the pyridoxine 5'-phosphate synthesizing enzyme.

Crystal structure of cystalysin from Treponema denticola: a pyridoxal 5'-phosphate-dependent protein acting as a haemolytic enzyme.

Crystal structure of the cystine C-S lyase from Synechocystis: stabilization of cysteine persulfide for FeS cluster biosynthesis.

X-ray structure of MalY from Escherichia coli: a pyridoxal 5'-phosphate-dependent enzyme acting as a modulator in mal gene expression.

A new mechanism for the control of a prokaryotic transcriptional regulator: antagonistic binding of positive and negative effectors.

Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly.

Cloning, purification and characterisation of cystathionine gamma-synthase from Nicotiana tabacum.

The crystal structure of cystathionine gamma-synthase from Nicotiana tabacum reveals its substrate and reaction specificity.

Characterization of recombinant Arabidopsis thaliana threonine synthase.

Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration.

Crystal structure of Escherichia coli cystathionine gamma-synthase at 1.5 A resolution.

Slow-binding inhibition of Escherichia coli cystathionine beta-lyase by L-aminoethoxyvinylglycine: a kinetic and X-ray study.

Cloning, purification, crystallization, and preliminary X-ray diffraction analysis of cystathionine gamma-synthase from E. coli.

Mode of action of cystathionine beta-lyase.

Glutaconate CoA-transferase from Acidaminococcus fermentans: the crystal structure reveals homology with other CoA-transferases.

Crystal structure of the pyridoxal-5'-phosphate dependent cystathionine beta-lyase from Escherichia coli at 1.83 A.

Cloning, purification, and crystallization of Escherichia coli cystathionine beta-lyase.

Involvement of Tyr24 and Trp108 in substrate binding and substrate specificity of glycolate oxidase.

Specific inhibition of transsulfuration enzymes.