Tim Clausen

RNA specificity and regulation of catalysis in the eukaryotic polynucleotide kinase Clp1.

Structural basis for recognizing phosphoarginine and evolving residue-specific protein phosphatases in gram-positive bacteria.

The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans.

CtpB assembles a gated protease tunnel regulating cell-cell signaling during spore formation in Bacillus subtilis.

A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors.

Human high temperature requirement serine protease A1 (HTRA1) degrades tau protein aggregates.

Stress-induced GSK3 regulates the redox stress response by phosphorylating glucose-6-phosphate dehydrogenase in Arabidopsis.

Newly folded substrates inside the molecular cage of the HtrA chaperone DegQ.

Mutations in the β-tubulin gene TUBB5 cause microcephaly with structural brain abnormalities.

Substrate-induced remodeling of the active site regulates human HTRA1 activity.

Augmenting β-augmentation: structural basis of how BamB binds BamA and may support folding of outer membrane proteins.

HTRA proteases: regulated proteolysis in protein quality control.

Molecular adaptation of the DegQ protease to exert protein quality control in the bacterial cell envelope.

Structural adaptation of the plant protease Deg1 to repair photosystem II during light exposure.

Protein quality control in the bacterial periplasm.

Conversion of a regulatory into a degradative protease.

Molecular transformers in the cell: lessons learned from the DegP protease-chaperone.

HtrA proteases have a conserved activation mechanism that can be triggered by distinct molecular cues.

Stoichiometry determination of the MP1-p14 complex using a novel and cost-efficient method to produce an equimolar mixture of standard peptides.

Structural basis of substrate specificity of plant 12-oxophytodienoate reductases.

Structure, function and regulation of the conserved serine proteases DegP and DegS of Escherichia coli.

Peptidic small molecule activators of the stress sensor DegS.

Selectivity profiling of DegP substrates and inhibitors.

McsB is a protein arginine kinase that phosphorylates and inhibits the heat-shock regulator CtsR.

Interplay of PDZ and protease domain of DegP ensures efficient elimination of misfolded proteins.

Allosteric activation of HtrA protease DegP by stress signals during bacterial protein quality control.

Regulation of the sigmaE stress response by DegS: how the PDZ domain keeps the protease inactive in the resting state and allows integration of different OMP-derived stress signals upon folding stress.

Crystal structure of 12-oxophytodienoate reductase 3 from tomato: self-inhibition by dimerization.

The role of human HtrA1 in arthritic disease.

Implications of the serine protease HtrA1 in amyloid precursor protein processing.

Molecular machines for protein degradation.

TIS7 regulation of the beta-catenin/Tcf-4 target gene osteopontin (OPN) is histone deacetylase-dependent.

The 1.3 A crystal structure of the flavoprotein YqjM reveals a novel class of Old Yellow Enzymes.

Structural and functional impairment of an Old Yellow Enzyme homologue upon affinity tag incorporation.

Muscle regeneration and myogenic differentiation defects in mice lacking TIS7.

Crystal structure of the p14/MP1 scaffolding complex: how a twin couple attaches mitogen-activated protein kinase signaling to late endosomes.

Proteolysis as a regulatory mechanism.

Crystal structure of the DegS stress sensor: How a PDZ domain recognizes misfolded protein and activates a protease.

Snapshots of the cystine lyase C-DES during catalysis. Studies in solution and in the crystalline state.

Molecular shredders: how proteasomes fulfill their role.

Spectroscopic and kinetic analyses reveal the pyridoxal 5'-phosphate binding mode and the catalytic features of Treponema denticola cystalysin.

Enzyme-ligand complexes of pyridoxine 5'-phosphate synthase: implications for substrate binding and catalysis.

Structure and function of threonine synthase from yeast.

Crystal structure of DegP (HtrA) reveals a new protease-chaperone machine.

The HtrA family of proteases: implications for protein composition and cell fate.

The three-dimensional structure of cystathionine beta-lyase from Arabidopsis and its substrate specificity.

Structural basis for the function of pyridoxine 5'-phosphate synthase.

Crystal structures of cystathionine gamma-synthase inhibitor complexes rationalize the increased affinity of a novel inhibitor.

X-ray structure of 12-oxophytodienoate reductase 1 provides structural insight into substrate binding and specificity within the family of OYE.

Crystal structure of transcription factor MalT domain III: a novel helix repeat fold implicated in regulated oligomerization.

Specific inhibition of transsulfuration enzymes.

X-ray structure of MalY from Escherichia coli: a pyridoxal 5'-phosphate-dependent enzyme acting as a modulator in mal gene expression.

Crystal structure of the cystine C-S lyase from Synechocystis: stabilization of cysteine persulfide for FeS cluster biosynthesis.

Crystallization and preliminary X-ray crystallographic analysis of PdxJ, the pyridoxine 5'-phosphate synthesizing enzyme.

Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly.

Crystal structure of cystalysin from Treponema denticola: a pyridoxal 5'-phosphate-dependent protein acting as a haemolytic enzyme.

A new mechanism for the control of a prokaryotic transcriptional regulator: antagonistic binding of positive and negative effectors.

Characterization of recombinant Arabidopsis thaliana threonine synthase.

Cloning, purification and characterisation of cystathionine gamma-synthase from Nicotiana tabacum.

Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration.

The crystal structure of cystathionine gamma-synthase from Nicotiana tabacum reveals its substrate and reaction specificity.

Crystal structure of Escherichia coli cystathionine gamma-synthase at 1.5 A resolution.

Mode of action of cystathionine beta-lyase.

Glutaconate CoA-transferase from Acidaminococcus fermentans: the crystal structure reveals homology with other CoA-transferases.

Slow-binding inhibition of Escherichia coli cystathionine beta-lyase by L-aminoethoxyvinylglycine: a kinetic and X-ray study.

Cloning, purification, crystallization, and preliminary X-ray diffraction analysis of cystathionine gamma-synthase from E. coli.

Crystal structure of the pyridoxal-5'-phosphate dependent cystathionine beta-lyase from Escherichia coli at 1.83 A.

Cloning, purification, and crystallization of Escherichia coli cystathionine beta-lyase.

Involvement of Tyr24 and Trp108 in substrate binding and substrate specificity of glycolate oxidase.