Publications
2025
Protein waste turned into antibiotics as a defence strategy of human cells.
Nature.Convergence of orphan quality control pathways at a ubiquitin chain-elongating ligase.
Corresponding preprint Mol Cell. 85(4):815-828.e10ATP functions as a pathogen-associated molecular pattern to activate the E3 ubiquitin ligase RNF213.
Nat Commun. 16(1):4414A split-site E3 ligase mechanism enables ZNFX1 to ubiquitinate and cluster single-stranded RNA into ubiquitin-coated nucleoprotein particles.
Cell.Architecture of the UBR4 complex, a giant E4 ligase central to eukaryotic protein quality control.
Science. 389(6763):909-914TRIM52 maintains cellular fitness and is under tight proteolytic control by multiple giant E3 ligases.
Nat Commun. 16(1):38942024
Homo-BacPROTAC-induced degradation of ClpC1 as a strategy against drug-resistant mycobacteria.
Nat Commun. 15(1):2005UNC-45 assisted myosin folding depends on a conserved FX3HY motif implicated in Freeman Sheldon Syndrome.
Nat Commun. 15(1):6272Rational correction of pathogenic conformational defects in HTRA1.
Nat Commun. 15(1):5944TRIM52 is a primate-specific player in the DNA repair process under tight proteolytic control by a triad of giant E3 ligases
bioRxiv. 10.1101/2024.05.16.594269Multiple ubiquitin ligases protect human genome integrity by targeting cancer-associated APOBEC3 deaminases for degradation
bioRxiv. 10.1101/2024.04.23.590688Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity
bioRxiv. 2024.04.23.5906882023
Structural basis for regulation of apoptosis and autophagy by the BIRC6/SMAC complex.
Science. 379(6637):1117-1123Shuffled ATG8 interacting motifs form an ancestral bridge between UFMylation and autophagy.
EMBO J. :e112053HUWE1 controls tristetraprolin proteasomal degradation by regulating its phosphorylation.
Corresponding preprint Elife. 12Clp-targeting BacPROTACs impair mycobacterial proteostasis and survival.
Cell. 186(10):2176-2192.e222022
BacPROTACs mediate targeted protein degradation in bacteria.
Corresponding preprint Cell. 185(13):2338-2353.e18An allosteric HTRA1-calpain 2 complex with restricted activation profile.
Proc Natl Acad Sci U S A. 119(14):e2113520119Moyamoya disease: diagnosis and interventions.
Lancet Neurol.Targeted protein degradation: from small molecules to complex organelles-a Keystone Symposia report.
Ann N Y Acad Sci. 1510(1):79-99Potent Bactericidal Antimycobacterials Targeting the Chaperone ClpC1 Based on the Depsipeptide Natural Products Ecumicin and Ohmyungsamycin A.
J Med Chem. 65(6):4893-49082021
The oxidoreductase PYROXD1 uses NAD(P)+ as an antioxidant to sustain tRNA ligase activity in pre-tRNA splicing and unfolded protein response.
Mol Cell. 81(12):2520-2532.e16E3 ubiquitin ligase RNF213 employs a non-canonical zinc finger active site and is allosterically regulated by ATP
bioRxiv. 2021.05.10.443411Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq.
Nat Commun. 12(1):3132The linear ubiquitin chain assembly complex (LUBAC) generates heterotypic ubiquitin chains.
Corresponding preprint Elife. 10McsB forms a gated kinase chamber to mark aberrant bacterial proteins for degradation.
Corresponding preprint Elife. 10HUWE1 employs a giant substrate-binding ring to feed and regulate its HECT E3 domain.
Nat Chem Biol.2020
Moyamoya disease factor RNF213 is a giant E3 ligase with a dynein-like core and a distinct ubiquitin-transfer mechanism.
Elife. 9Activation by substoichiometric inhibition.
Proc Natl Acad Sci U S A. 117(3):1414-1418A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress.
Elife. 9Native Mass Spectrometry Can Effectively Predict PROTAC Efficacy.
ACS Cent Sci. 6(7):1223-12302019
Structure of McsB, a protein kinase for regulated arginine phosphorylation.
Nat Chem Biol. 15(5):510-518Shaping Striated Muscles with Ubiquitin Proteasome System in Health and Disease.
Trends Mol Med. 25(9):760-774Molecular features of the UNC-45 chaperone critical for binding and folding muscle myosin.
Nat Commun. 10(1):47812018
UFD-2 is an adaptor-assisted E3 ligase targeting unfolded proteins.
Nat Commun. 9(1):4842017
The crystal structure of Deg9 reveals a novel octameric-type HtrA protease.
Nat Plants. 3(12):973-9822016
Chemical Biology Interrogates Protein Arginine Phosphorylation.
Cell Chem Biol. 23(8):888-90Arginine phosphorylation marks proteins for degradation by a Clp protease.
Nature. 539(7627):48-53Structural mechanism for the recognition and ubiquitination of a single nucleosome residue by Rad6-Bre1.
Proc Natl Acad Sci U S A. 113(38):10553-8Structural basis for the disaggregase activity and regulation of Hsp104.
Elife. 52015
The Nuclear Pore-Associated TREX-2 Complex Employs Mediator to Regulate Gene Expression.
Cell. 162(5):1016-28Determinants of amyloid fibril degradation by the PDZ protease HTRA1.
Nat Chem Biol. 11(11):862-92014
RNA specificity and regulation of catalysis in the eukaryotic polynucleotide kinase Clp1.
Mol Cell. 54(6):975-86Quantitative phosphoproteomics reveals the role of protein arginine phosphorylation in the bacterial stress response.
Mol Cell Proteomics. 13(2):537-50Human CLP1 mutations alter tRNA biogenesis, affecting both peripheral and central nervous system function.
Cell. 157(3):636-50Versatile in vitro system to study translocation and functional integration of bacterial outer membrane proteins.
Nat Commun. 5:5396Chasing Phosphoarginine Proteins: Development of a Selective Enrichment Method Using a Phosphatase Trap.
Mol Cell Proteomics. 13(8):1953-642013
CtpB assembles a gated protease tunnel regulating cell-cell signaling during spore formation in Bacillus subtilis.
Cell. 155(3):647-58Structural basis for recognizing phosphoarginine and evolving residue-specific protein phosphatases in gram-positive bacteria.
Cell Rep. 3(6):1832-9A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors.
EMBO J. 32(3):409-23The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans.
Cell. 152(1-2):183-952012
Human high temperature requirement serine protease A1 (HTRA1) degrades tau protein aggregates.
J Biol Chem. 287(25):20931-41Stress-induced GSK3 regulates the redox stress response by phosphorylating glucose-6-phosphate dehydrogenase in Arabidopsis.
Plant Cell. 24(8):3380-92Newly folded substrates inside the molecular cage of the HtrA chaperone DegQ.
Nat Struct Mol Biol. 19(2):152-7Mutations in the β-tubulin gene TUBB5 cause microcephaly with structural brain abnormalities.
Cell Rep. 2(6):1554-622011
Molecular adaptation of the DegQ protease to exert protein quality control in the bacterial cell envelope.
J Biol Chem. 286(35):30680-30690Structural adaptation of the plant protease Deg1 to repair photosystem II during light exposure.
Nat Struct Mol Biol. 18(6):728-31HTRA proteases: regulated proteolysis in protein quality control.
Nat Rev Mol Cell Biol. 12(3):152-62Augmenting β-augmentation: structural basis of how BamB binds BamA and may support folding of outer membrane proteins.
J Mol Biol. 406(5):659-66Substrate-induced remodeling of the active site regulates human HTRA1 activity.
Nat Struct Mol Biol. 18(3):386-8Protein quality control in the bacterial periplasm.
Annu Rev Microbiol. 65:149-682010
Molecular transformers in the cell: lessons learned from the DegP protease-chaperone.
Curr Opin Struct Biol. 20(2):253-8Conversion of a regulatory into a degradative protease.
J Mol Biol. 397(4):957-66HtrA proteases have a conserved activation mechanism that can be triggered by distinct molecular cues.
Nat Struct Mol Biol. 17(7):844-522009
Selectivity profiling of DegP substrates and inhibitors.
Bioorg Med Chem. 17(7):2920-4McsB is a protein arginine kinase that phosphorylates and inhibits the heat-shock regulator CtsR.
Science. 324(5932):1323-7Peptidic small molecule activators of the stress sensor DegS.
Mol Biosyst. 5(9):980-5Structure, function and regulation of the conserved serine proteases DegP and DegS of Escherichia coli.
Res Microbiol. 160(9):660-6Structural basis of substrate specificity of plant 12-oxophytodienoate reductases.
J Mol Biol. 392(5):1266-77Stoichiometry determination of the MP1-p14 complex using a novel and cost-efficient method to produce an equimolar mixture of standard peptides.
Anal Chem. 81(24):10254-612008
Interplay of PDZ and protease domain of DegP ensures efficient elimination of misfolded proteins.
Proc Natl Acad Sci U S A. 105(22):7702-7Structural basis for the regulated protease and chaperone function of DegP.
Nature. 453(7197):885-90Allosteric activation of HtrA protease DegP by stress signals during bacterial protein quality control.
Angew Chem Int Ed Engl. 47(7):1332-42007
Regulation of the sigmaE stress response by DegS: how the PDZ domain keeps the protease inactive in the resting state and allows integration of different OMP-derived stress signals upon folding stress.
Genes Dev. 21(20):2659-702006
Crystal structure of 12-oxophytodienoate reductase 3 from tomato: self-inhibition by dimerization.
Proc Natl Acad Sci U S A. 103(39):14337-42The role of human HtrA1 in arthritic disease.
J Biol Chem. 281(10):6124-92005
TIS7 regulation of the beta-catenin/Tcf-4 target gene osteopontin (OPN) is histone deacetylase-dependent.
J Biol Chem. 280(48):39795-801The 1.3 A crystal structure of the flavoprotein YqjM reveals a novel class of Old Yellow Enzymes.
J Biol Chem. 280(30):27904-13Molecular machines for protein degradation.
Chembiochem. 6(2):222-56Implications of the serine protease HtrA1 in amyloid precursor protein processing.
Proc Natl Acad Sci U S A. 102(17):6021-62004
Crystal structure of the DegS stress sensor: How a PDZ domain recognizes misfolded protein and activates a protease.
Cell. 117(4):483-94Structural and functional impairment of an Old Yellow Enzyme homologue upon affinity tag incorporation.
Protein Expr Purif. 36(2):280-91Muscle regeneration and myogenic differentiation defects in mice lacking TIS7.
Mol Cell Biol. 24(8):3514-25Crystal structure of the p14/MP1 scaffolding complex: how a twin couple attaches mitogen-activated protein kinase signaling to late endosomes.
Proc Natl Acad Sci U S A. 101(30):10984-92003
Molecular shredders: how proteasomes fulfill their role.
Curr Opin Struct Biol. 13(6):665-73Snapshots of the cystine lyase C-DES during catalysis. Studies in solution and in the crystalline state.
J Biol Chem. 278(1):357-652002
The HtrA family of proteases: implications for protein composition and cell fate.
Mol Cell. 10(3):443-55Crystal structure of DegP (HtrA) reveals a new protease-chaperone machine.
Nature. 416(6879):455-9Enzyme-ligand complexes of pyridoxine 5'-phosphate synthase: implications for substrate binding and catalysis.
J Mol Biol. 321(4):601-12Spectroscopic and kinetic analyses reveal the pyridoxal 5'-phosphate binding mode and the catalytic features of Treponema denticola cystalysin.
Biochemistry. 41(29):9153-64Structure and function of threonine synthase from yeast.
J Biol Chem. 277(14):12396-4052001
The three-dimensional structure of cystathionine beta-lyase from Arabidopsis and its substrate specificity.
Plant Physiol. 126(2):631-42Crystal structures of cystathionine gamma-synthase inhibitor complexes rationalize the increased affinity of a novel inhibitor.
J Mol Biol. 311(4):789-801Crystal structure of transcription factor MalT domain III: a novel helix repeat fold implicated in regulated oligomerization.
Structure. 9(11):1051-60X-ray structure of 12-oxophytodienoate reductase 1 provides structural insight into substrate binding and specificity within the family of OYE.
Structure. 9(5):419-29Structural basis for the function of pyridoxine 5'-phosphate synthase.
Structure. 9(3):245-532000
Specific inhibition of transsulfuration enzymes.
Rec Res Developm Biochem. 2:191-207Crystallization and preliminary X-ray crystallographic analysis of PdxJ, the pyridoxine 5'-phosphate synthesizing enzyme.
Acta Crystallogr D Biol Crystallogr. 56(Pt 8):1045-8Crystal structure of the cystine C-S lyase from Synechocystis: stabilization of cysteine persulfide for FeS cluster biosynthesis.
Proc Natl Acad Sci U S A. 97(8):3856-61X-ray structure of MalY from Escherichia coli: a pyridoxal 5'-phosphate-dependent enzyme acting as a modulator in mal gene expression.
EMBO J. 19(5):831-42A new mechanism for the control of a prokaryotic transcriptional regulator: antagonistic binding of positive and negative effectors.
Mol Microbiol. 35(4):765-76Crystal structure of cystalysin from Treponema denticola: a pyridoxal 5'-phosphate-dependent protein acting as a haemolytic enzyme.
EMBO J. 19(13):3168-78Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly.
J Mol Biol. 297(2):451-641999
The crystal structure of cystathionine gamma-synthase from Nicotiana tabacum reveals its substrate and reaction specificity.
J Mol Biol. 290(5):983-96Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration.
J Biol Chem. 274(18):12675-84Cloning, purification and characterisation of cystathionine gamma-synthase from Nicotiana tabacum.
Biol Chem. 380(10):1237-42Characterization of recombinant Arabidopsis thaliana threonine synthase.
Eur J Biochem. 263(1):212-211998
Crystal structure of Escherichia coli cystathionine gamma-synthase at 1.5 A resolution.
EMBO J. 17(23):6827-381997
Cloning, purification, crystallization, and preliminary X-ray diffraction analysis of cystathionine gamma-synthase from E. coli.
FEBS Lett. 414(3):492-6Glutaconate CoA-transferase from Acidaminococcus fermentans: the crystal structure reveals homology with other CoA-transferases.
Structure. 5(3):415-26Mode of action of cystathionine beta-lyase.
Biol Chem. 378(3-4):321-6Slow-binding inhibition of Escherichia coli cystathionine beta-lyase by L-aminoethoxyvinylglycine: a kinetic and X-ray study.
Biochemistry. 36(41):12633-431996
Cloning, purification, and crystallization of Escherichia coli cystathionine beta-lyase.
FEBS Lett. 379(1):94-6Crystal structure of the pyridoxal-5'-phosphate dependent cystathionine beta-lyase from Escherichia coli at 1.83 A.
J Mol Biol. 262(2):202-241995
Involvement of Tyr24 and Trp108 in substrate binding and substrate specificity of glycolate oxidase.
Eur J Biochem. 228(2):408-16