Tim Clausen

Protein waste turned into antibiotics as a defence strategy of human cells.

Convergence of orphan quality control pathways at a ubiquitin chain-elongating ligase.

ATP functions as a pathogen-associated molecular pattern to activate the E3 ubiquitin ligase RNF213.

A split-site E3 ligase mechanism enables ZNFX1 to ubiquitinate and cluster single-stranded RNA into ubiquitin-coated nucleoprotein particles.

Architecture of the UBR4 complex, a giant E4 ligase central to eukaryotic protein quality control.

TRIM52 maintains cellular fitness and is under tight proteolytic control by multiple giant E3 ligases.

Homo-BacPROTAC-induced degradation of ClpC1 as a strategy against drug-resistant mycobacteria.

UNC-45 assisted myosin folding depends on a conserved FX3HY motif implicated in Freeman Sheldon Syndrome.

Rational correction of pathogenic conformational defects in HTRA1.

TRIM52 is a primate-specific player in the DNA repair process under tight proteolytic control by a triad of giant E3 ligases

Multiple ubiquitin ligases protect human genome integrity by targeting cancer-associated APOBEC3 deaminases for degradation

Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity

Structural basis for regulation of apoptosis and autophagy by the BIRC6/SMAC complex.

Shuffled ATG8 interacting motifs form an ancestral bridge between UFMylation and autophagy.

HUWE1 controls tristetraprolin proteasomal degradation by regulating its phosphorylation.

Clp-targeting BacPROTACs impair mycobacterial proteostasis and survival.

BacPROTACs mediate targeted protein degradation in bacteria.

An allosteric HTRA1-calpain 2 complex with restricted activation profile.

Moyamoya disease: diagnosis and interventions.

Targeted protein degradation: from small molecules to complex organelles-a Keystone Symposia report.

Potent Bactericidal Antimycobacterials Targeting the Chaperone ClpC1 Based on the Depsipeptide Natural Products Ecumicin and Ohmyungsamycin A.

The oxidoreductase PYROXD1 uses NAD(P)+ as an antioxidant to sustain tRNA ligase activity in pre-tRNA splicing and unfolded protein response.

E3 ubiquitin ligase RNF213 employs a non-canonical zinc finger active site and is allosterically regulated by ATP

Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq.

The linear ubiquitin chain assembly complex (LUBAC) generates heterotypic ubiquitin chains.

McsB forms a gated kinase chamber to mark aberrant bacterial proteins for degradation.

HUWE1 employs a giant substrate-binding ring to feed and regulate its HECT E3 domain.

Moyamoya disease factor RNF213 is a giant E3 ligase with a dynein-like core and a distinct ubiquitin-transfer mechanism.

Activation by substoichiometric inhibition.

A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress.

Native Mass Spectrometry Can Effectively Predict PROTAC Efficacy.

Structure of McsB, a protein kinase for regulated arginine phosphorylation.

Shaping Striated Muscles with Ubiquitin Proteasome System in Health and Disease.

Molecular features of the UNC-45 chaperone critical for binding and folding muscle myosin.

UFD-2 is an adaptor-assisted E3 ligase targeting unfolded proteins.

The crystal structure of Deg9 reveals a novel octameric-type HtrA protease.

Chemical Biology Interrogates Protein Arginine Phosphorylation.

Arginine phosphorylation marks proteins for degradation by a Clp protease.

Structural mechanism for the recognition and ubiquitination of a single nucleosome residue by Rad6-Bre1.

Structural basis for the disaggregase activity and regulation of Hsp104.

The Nuclear Pore-Associated TREX-2 Complex Employs Mediator to Regulate Gene Expression.

Determinants of amyloid fibril degradation by the PDZ protease HTRA1.

RNA specificity and regulation of catalysis in the eukaryotic polynucleotide kinase Clp1.

Quantitative phosphoproteomics reveals the role of protein arginine phosphorylation in the bacterial stress response.

Human CLP1 mutations alter tRNA biogenesis, affecting both peripheral and central nervous system function.

Versatile in vitro system to study translocation and functional integration of bacterial outer membrane proteins.

Chasing Phosphoarginine Proteins: Development of a Selective Enrichment Method Using a Phosphatase Trap.

Myosin chaperones.

CtpB assembles a gated protease tunnel regulating cell-cell signaling during spore formation in Bacillus subtilis.

Structural basis for recognizing phosphoarginine and evolving residue-specific protein phosphatases in gram-positive bacteria.

A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors.

The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans.

Human high temperature requirement serine protease A1 (HTRA1) degrades tau protein aggregates.

Stress-induced GSK3 regulates the redox stress response by phosphorylating glucose-6-phosphate dehydrogenase in Arabidopsis.

Newly folded substrates inside the molecular cage of the HtrA chaperone DegQ.

Mutations in the β-tubulin gene TUBB5 cause microcephaly with structural brain abnormalities.

Molecular adaptation of the DegQ protease to exert protein quality control in the bacterial cell envelope.

Structural adaptation of the plant protease Deg1 to repair photosystem II during light exposure.

HTRA proteases: regulated proteolysis in protein quality control.

Augmenting β-augmentation: structural basis of how BamB binds BamA and may support folding of outer membrane proteins.

Substrate-induced remodeling of the active site regulates human HTRA1 activity.

Protein quality control in the bacterial periplasm.

Molecular transformers in the cell: lessons learned from the DegP protease-chaperone.

Conversion of a regulatory into a degradative protease.

HtrA proteases have a conserved activation mechanism that can be triggered by distinct molecular cues.

Selectivity profiling of DegP substrates and inhibitors.

McsB is a protein arginine kinase that phosphorylates and inhibits the heat-shock regulator CtsR.

Peptidic small molecule activators of the stress sensor DegS.

Structure, function and regulation of the conserved serine proteases DegP and DegS of Escherichia coli.

Structural basis of substrate specificity of plant 12-oxophytodienoate reductases.

Stoichiometry determination of the MP1-p14 complex using a novel and cost-efficient method to produce an equimolar mixture of standard peptides.

Interplay of PDZ and protease domain of DegP ensures efficient elimination of misfolded proteins.

Structural basis for the regulated protease and chaperone function of DegP.

Allosteric activation of HtrA protease DegP by stress signals during bacterial protein quality control.

Regulation of the sigmaE stress response by DegS: how the PDZ domain keeps the protease inactive in the resting state and allows integration of different OMP-derived stress signals upon folding stress.

Crystal structure of 12-oxophytodienoate reductase 3 from tomato: self-inhibition by dimerization.

The role of human HtrA1 in arthritic disease.

TIS7 regulation of the beta-catenin/Tcf-4 target gene osteopontin (OPN) is histone deacetylase-dependent.

The 1.3 A crystal structure of the flavoprotein YqjM reveals a novel class of Old Yellow Enzymes.

Molecular machines for protein degradation.

Implications of the serine protease HtrA1 in amyloid precursor protein processing.

Crystal structure of the DegS stress sensor: How a PDZ domain recognizes misfolded protein and activates a protease.

Structural and functional impairment of an Old Yellow Enzyme homologue upon affinity tag incorporation.

Muscle regeneration and myogenic differentiation defects in mice lacking TIS7.

Crystal structure of the p14/MP1 scaffolding complex: how a twin couple attaches mitogen-activated protein kinase signaling to late endosomes.

Proteolysis as a regulatory mechanism.

Molecular shredders: how proteasomes fulfill their role.

Snapshots of the cystine lyase C-DES during catalysis. Studies in solution and in the crystalline state.

The HtrA family of proteases: implications for protein composition and cell fate.

Crystal structure of DegP (HtrA) reveals a new protease-chaperone machine.

Enzyme-ligand complexes of pyridoxine 5'-phosphate synthase: implications for substrate binding and catalysis.

Spectroscopic and kinetic analyses reveal the pyridoxal 5'-phosphate binding mode and the catalytic features of Treponema denticola cystalysin.

Structure and function of threonine synthase from yeast.

The three-dimensional structure of cystathionine beta-lyase from Arabidopsis and its substrate specificity.

Crystal structures of cystathionine gamma-synthase inhibitor complexes rationalize the increased affinity of a novel inhibitor.

Crystal structure of transcription factor MalT domain III: a novel helix repeat fold implicated in regulated oligomerization.

X-ray structure of 12-oxophytodienoate reductase 1 provides structural insight into substrate binding and specificity within the family of OYE.

Structural basis for the function of pyridoxine 5'-phosphate synthase.

Specific inhibition of transsulfuration enzymes.

Crystallization and preliminary X-ray crystallographic analysis of PdxJ, the pyridoxine 5'-phosphate synthesizing enzyme.

Crystal structure of the cystine C-S lyase from Synechocystis: stabilization of cysteine persulfide for FeS cluster biosynthesis.

X-ray structure of MalY from Escherichia coli: a pyridoxal 5'-phosphate-dependent enzyme acting as a modulator in mal gene expression.

A new mechanism for the control of a prokaryotic transcriptional regulator: antagonistic binding of positive and negative effectors.

Crystal structure of cystalysin from Treponema denticola: a pyridoxal 5'-phosphate-dependent protein acting as a haemolytic enzyme.

Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly.

The crystal structure of cystathionine gamma-synthase from Nicotiana tabacum reveals its substrate and reaction specificity.

Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration.

Cloning, purification and characterisation of cystathionine gamma-synthase from Nicotiana tabacum.

Characterization of recombinant Arabidopsis thaliana threonine synthase.

Crystal structure of Escherichia coli cystathionine gamma-synthase at 1.5 A resolution.

Cloning, purification, crystallization, and preliminary X-ray diffraction analysis of cystathionine gamma-synthase from E. coli.

Glutaconate CoA-transferase from Acidaminococcus fermentans: the crystal structure reveals homology with other CoA-transferases.

Mode of action of cystathionine beta-lyase.

Slow-binding inhibition of Escherichia coli cystathionine beta-lyase by L-aminoethoxyvinylglycine: a kinetic and X-ray study.

Cloning, purification, and crystallization of Escherichia coli cystathionine beta-lyase.

Crystal structure of the pyridoxal-5'-phosphate dependent cystathionine beta-lyase from Escherichia coli at 1.83 A.

Involvement of Tyr24 and Trp108 in substrate binding and substrate specificity of glycolate oxidase.