Jan-Michael Peters

SMC motor proteins extrude DNA asymmetrically and can switch directions.

Quantitative imaging of loop extruders rebuilding interphase genome architecture after mitosis.

Cohesin positions the epigenetic reader Phf2 within the genome.

PDS5 proteins control genome architecture by limiting the lifetime of cohesin-NIPBL complexes

The mitotic STAG3-cohesin complex shapes male germline nucleome.

NIPBL and STAG1 enable loop extrusion by providing differential DNA-cohesin affinity.

Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ.

Cohesin supercoils DNA during loop extrusion.

Sororin is an evolutionary conserved antagonist of WAPL.

All eukaryotic SMC proteins induce a twist of -0.6 at each DNA loop extrusion step.

Transcription shapes 3D chromatin organization by interacting with loop extrusion.

Igh and Igk loci use different folding principles for V gene recombination due to distinct chromosomal architectures of pro-B and pre-B cells.

CTCF is a DNA-tension-dependent barrier to cohesin-mediated loop extrusion.

Testing pseudotopological and nontopological models for SMC-driven DNA loop extrusion against roadblock-traversal experiments.

Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase.

Cohesin mediates DNA loop extrusion and sister chromatid cohesion by distinct mechanisms.

How do molecular motors fold the genome?

Cohesin and CTCF do not assemble TADs in Xenopus sperm and male pronuclei.

MCM complexes are barriers that restrict cohesin-mediated loop extrusion.

Nucleome programming is required for the foundation of totipotency in mammalian germline development.

Cornelia de Lange syndrome mutations in NIPBL can impair cohesin-mediated DNA loop extrusion.

Sororin is an evolutionary conserved antagonist of WAPL

Cohesin mediates DNA loop extrusion and sister chromatid cohesion by distinct mechanisms

SMC complexes can traverse physical roadblocks bigger than their ring size.

How DNA loop extrusion mediated by cohesin enables V(D)J recombination.

Genome folding through loop extrusion by SMC complexes.

Cohesin mediates DNA loop extrusion by a "swing and clamp" mechanism.

Angelika Amon (1967-2020).

Visualization of loop extrusion by DNA nanoscale tracing in single human cells

Wapl releases Scc1-cohesin and regulates chromosome structure and segregation in mouse oocytes.

Wapl repression by Pax5 promotes V gene recombination by Igh loop extrusion.

Quantifying the heterogeneity of macromolecular machines by mass photometry.

STAG1 vulnerabilities for exploiting cohesin synthetic lethality in STAG2-deficient cancers.

Ubiquitin chain-elongating enzyme UBE2S activates the RING E3 ligase APC/C for substrate priming.

ESCO1 and CTCF enable formation of long chromatin loops by protecting cohesinSTAG1 from WAPL.

PDS5 proteins are required for proper cohesin dynamics and participate in replication fork protection.

Conformation of sister chromatids in the replicated human genome.

Cohesin-Dependent and -Independent Mechanisms Mediate Chromosomal Contacts between Promoters and Enhancers.

Posing the APC/C E3 Ubiquitin Ligase to Orchestrate Cell Division.

Absolute quantification of cohesin, CTCF and their regulators in human cells.

Protein engineering of a ubiquitin-variant inhibitor of APC/C identifies a cryptic K48 ubiquitin chain binding site.

Topoisomerase II-Induced Chromosome Breakage and Translocation Is Determined by Chromosome Architecture and Transcriptional Activity.

Self-organization of parS centromeres by the ParB CTP hydrolase.

DNA loop extrusion by human cohesin.

Werner syndrome helicase is a selective vulnerability of microsatellite instability-high tumor cells.

Dynamics of sister chromatid resolution during cell cycle progression.

Expressing Multi-subunit Complexes Using biGBac.

The replicative helicase MCM recruits cohesin acetyltransferase ESCO2 to mediate centromeric sister chromatid cohesion.

Analysis of chromosomes from mouse oocytes and mammalian cultured cells by light microscopy.

Experimental and computational framework for a dynamic protein atlas of human cell division.

Cohesin is positioned in mammalian genomes by transcription, CTCF and Wapl.

Synthetic lethality between the cohesin subunits STAG1 and STAG2 in diverse cancer contexts.

Topologically associating domains and chromatin loops depend on cohesin and are regulated by CTCF, WAPL, and PDS5 proteins.

A mechanism of cohesin-dependent loop extrusion organizes zygotic genome architecture.

BubR1 Promotes Bub3-Dependent APC/C Inhibition during Spindle Assembly Checkpoint Signaling.

Cryo-EM of Mitotic Checkpoint Complex-Bound APC/C Reveals Reciprocal and Conformational Regulation of Ubiquitin Ligation.

Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C.

Rapid movement and transcriptional re-localization of human cohesin on DNA.

Intact Cohesion, Anaphase, and Chromosome Segregation in Human Cells Harboring Tumor-Derived Mutations in STAG2.

biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes.

ARHGEF17 is an essential spindle assembly checkpoint factor that targets Mps1 to kinetochores.

Sororin actively maintains sister chromatid cohesion.

Mechanism of APC/CCDC20 activation by mitotic phosphorylation.

Measuring APC/C-Dependent Ubiquitylation In Vitro.

Topology and structure of an engineered human cohesin complex bound to Pds5B.

ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space.

The Deubiquitinase USP37 Regulates Chromosome Cohesion and Mitotic Progression.

Structure of an APC3-APC16 complex: insights into assembly of the anaphase-promoting complex/cyclosome.

RING E3 mechanism for ubiquitin ligation to a disordered substrate visualized for human anaphase-promoting complex.

Characterization of a DNA exit gate in the human cohesin ring.

Mechanism of polyubiquitination by human anaphase-promoting complex: RING repurposing for ubiquitin chain assembly.

SNW1 enables sister chromatid cohesion by mediating the splicing of sororin and APC2 pre-mRNAs.

Cohesin's ATPase activity couples cohesin loading onto DNA with Smc3 acetylation.

Electron microscopy structure of human APC/C(CDH1)-EMI1 reveals multimodal mechanism of E3 ligase shutdown.

Aurora B and Cdk1 mediate Wapl activation and release of acetylated cohesin from chromosomes by phosphorylating Sororin.

Wapl is an essential regulator of chromatin structure and chromosome segregation.

Cohesin acetyltransferase Esco2 is a cell viability factor and is required for cohesion in pericentric heterochromatin.

APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex.

The non-redundant function of cohesin acetyltransferase Esco2: some answers and new questions.

The many functions of cohesin--different rings to rule them all?

Sister chromatid cohesion.

Lesson from the stoichiometry determination of the cohesin complex: a short protease mediated elution increases the recovery from cross-linked antibody-conjugated beads.

Spatial exclusivity combined with positive and negative selection of phosphorylation motifs is the basis for context-dependent mitotic signaling.

Systematic phosphorylation analysis of human mitotic protein complexes.

Substrate binding on the APC/C occurs between the coactivator Cdh1 and the processivity factor Doc1.

Quantitative phospho-proteomics to investigate the polo-like kinase 1-dependent phospho-proteome.

Live-cell imaging RNAi screen identifies PP2A-B55alpha and importin-beta1 as key mitotic exit regulators in human cells.

A new acid mix enhances phosphopeptide enrichment on titanium- and zirconium dioxide for mapping of phosphorylation sites on protein complexes.

Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes.

Systematic analysis of human protein complexes identifies chromosome segregation proteins.

Sororin mediates sister chromatid cohesion by antagonizing Wapl.

The cohesin complex is required for the DNA damage-induced G2/M checkpoint in mammalian cells.

How cohesin and CTCF cooperate in regulating gene expression.

Cohesin acetylation: from antiestablishment to establishment.

Structure of the anaphase-promoting complex/cyclosome interacting with a mitotic checkpoint complex.

Preventing carryover of peptides and proteins in nano LC-MS separations.

Cohesin is required for higher-order chromatin conformation at the imprinted IGF2-H19 locus.

HAUS, the 8-subunit human Augmin complex, regulates centrosome and spindle integrity.

Polo on the Rise-from Mitotic Entry to Cytokinesis with Plk1.

Twenty years of cell-cycle conferences in Roscoff.

BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals.

Checkpoint control: the journey continues.

Polo and Aurora kinases: lessons derived from chemical biology.

The Suv39h-HP1 histone methylation pathway is dispensable for enrichment and protection of cohesin at centromeres in mammalian cells.

GraFix: sample preparation for single-particle electron cryomicroscopy.

The cohesin complex and its roles in chromosome biology.

Cohesin mediates transcriptional insulation by CCCTC-binding factor.

Molecular biology. How and when the genome sticks together.

Cell biology: the checkpoint brake relieved.

The complete removal of cohesin from chromosome arms depends on separase.

Titanium dioxide as a chemo-affinity solid phase in offline phosphopeptide chromatography prior to HPLC-MS/MS analysis.

A systematic comparative and structural analysis of protein phosphorylation sites based on the mtcPTM database.

Polo-like kinase 1 triggers the initiation of cytokinesis in human cells by promoting recruitment of the RhoGEF Ect2 to the central spindle.

Aurora B controls the association of condensin I but not condensin II with mitotic chromosomes.

Sororin is required for stable binding of cohesin to chromatin and for sister chromatid cohesion in interphase.

BI 2536, a potent and selective inhibitor of polo-like kinase 1, inhibits tumor growth in vivo.

The small-molecule inhibitor BI 2536 reveals novel insights into mitotic roles of polo-like kinase 1.

Checkpoint activation: don't get mad too much.

How APC/C orders destruction.

Wapl controls the dynamic association of cohesin with chromatin.

Cleaning of raw peptide MS/MS spectra: improved protein identification following deconvolution of multiply charged peaks, isotope clusters, and removal of background noise.

The anaphase promoting complex/cyclosome: a machine designed to destroy.

Cohesin and DNA damage repair.

Separase: a universal trigger for sister chromatid disjunction but not chromosome cycle progression.

Condensin I stabilizes chromosomes mechanically through a dynamic interaction in live cells.

Live-cell imaging reveals a stable cohesin-chromatin interaction after but not before DNA replication.

Methods to measure ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex.

Human Scc4 is required for cohesin binding to chromatin, sister-chromatid cohesion, and mitotic progression.

Cyclin degradation: don't mes(s) with meiosis.

Localization of the coactivator Cdh1 and the cullin subunit Apc2 in a cryo-electron microscopy model of vertebrate APC/C.

Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2.

Histone H3 serine 10 phosphorylation by Aurora B causes HP1 dissociation from heterochromatin.

The WD40 propeller domain of Cdh1 functions as a destruction box receptor for APC/C substrates.

Shugoshin prevents dissociation of cohesin from centromeres during mitosis in vertebrate cells.

Identification of cell cycle-dependent phosphorylation sites on the anaphase-promoting complex/cyclosome by mass spectrometry.

Large-scale purification of the vertebrate anaphase-promoting complex/cyclosome.

APC activators caught by their tails?

Distinct functions of condensin I and II in mitotic chromosome assembly.

APC/C and SCF: controlling each other and the cell cycle.

The anaphase promoting complex/cyclosome is recruited to centromeres by the spindle assembly checkpoint.

Regulation of sister chromatid cohesion between chromosome arms.

[Setting the stage for mitosis: kinase regulation of prophase].

The E2-C vihar is required for the correct spatiotemporal proteolysis of cyclin B and itself undergoes cyclical degradation.

Roles of polo-like kinase 1 in the assembly of functional mitotic spindles.

Mitotic entry: tipping the balance.

Emi1 proteolysis: how SCF(beta-Trcp1) helps to activate the anaphase-promoting complex.

Fine tuning of kinetochore function by phosphorylation.

Mitotic regulation of the human anaphase-promoting complex by phosphorylation.

The meiosis I-to-meiosis II transition in mouse oocytes requires separase activity.

Early mitotic degradation of the homeoprotein HOXC10 is potentially linked to cell cycle progression.

The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in maintaining the spindle assembly checkpoint.

Identification of a subunit of a novel Kleisin-beta/SMC complex as a potential substrate of protein phosphatase 2A.

TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1.

Anaphase-promoting complex.

Conspiracy to disarm APC in interphase.

The anaphase-promoting complex: proteolysis in mitosis and beyond.

Human securin proteolysis is controlled by the spindle checkpoint and reveals when the APC/C switches from activation by Cdc20 to Cdh1.

The dissociation of cohesin from chromosomes in prophase is regulated by Polo-like kinase.

Regulation of human separase by securin binding and autocleavage.

Cell cycle: Waiters serving the Destruction machinery.

Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex.

Scc1/Rad21/Mcd1 is required for sister chromatid cohesion and kinetochore function in vertebrate cells.

Crystal structure of the APC10/DOC1 subunit of the human anaphase-promoting complex.

Cohesin cleavage by separase required for anaphase and cytokinesis in human cells.

Securin is required for chromosomal stability in human cells.

A conserved cyclin-binding domain determines functional interplay between anaphase-promoting complex-Cdh1 and cyclin A-Cdk2 during cell cycle progression.

Three-dimensional structure of the anaphase-promoting complex.

Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint.

Splitting the chromosome: cutting the ties that bind sister chromatids.

Cullins and cell cycle control.

Two distinct pathways remove mammalian cohesin from chromosome arms in prophase and from centromeres in anaphase.

Characterization of vertebrate cohesin complexes and their regulation in prophase.

Nonperiodic activity of the human anaphase-promoting complex-Cdh1 ubiquitin ligase results in continuous DNA synthesis uncoupled from mitosis.

Cell cycle- and cell growth-regulated proteolysis of mammalian CDC6 is dependent on APC-CDH1.

The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex.

Splitting the chromosome: cutting the ties that bind sister chromatids.

Mitotic regulation of the APC activator proteins CDC20 and CDH1.

Accumulation of cyclin B1 requires E2F and cyclin-A-dependent rearrangement of the anaphase-promoting complex.

Expression of the CDH1-associated form of the anaphase-promoting complex in postmitotic neurons.

Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex.

Subunits and substrates of the anaphase-promoting complex.

Cell cycle control by ubiquitin-dependent proteolysis.

SCF and APC: the Yin and Yang of cell cycle regulated proteolysis.

Activation of the human anaphase-promoting complex by proteins of the CDC20/Fizzy family.

Regulation of the cyclin B degradation system by an inhibitor of mitotic proteolysis.

Identification of a cullin homology region in a subunit of the anaphase-promoting complex.

APC-mediated proteolysis of Ase1 and the morphogenesis of the mitotic spindle.

Topogenesis of a nucleolar protein: determination of molecular segments directing nucleolar association.

How proteolysis drives the cell cycle.

Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p.

Identification of BIME as a subunit of the anaphase-promoting complex.

Identification of a novel ubiquitin-conjugating enzyme involved in mitotic cyclin degradation.

A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B.

An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic Golgi fragments.

The formation of Golgi stacks from vesiculated Golgi membranes requires two distinct fusion events.

The ATPase activity of purified CDC48p from Saccharomyces cerevisiae shows complex dependence on ATP-, ADP-, and NADH-concentrations and is completely inhibited by NEM.

Maintenance of cell-type-specific cytoskeletal character in epithelial cells out of epithelial context: cytokeratins and other cytoskeletal proteins in the rests of Malassez of the periodontal ligament.

Proteasomes: protein degradation machines of the cell.

Distinct 19 S and 20 S subcomplexes of the 26 S proteasome and their distribution in the nucleus and the cytoplasm.

Structural features of the 26 S proteasome complex.

Ubiquitous soluble Mg(2+)-ATPase complex. A structural study.

Ultrastructure of the approximately 26S complex containing the approximately 20S cylinder particle (multicatalytic proteinase/proteasome).

An abundant and ubiquitous homo-oligomeric ring-shaped ATPase particle related to the putative vesicle fusion proteins Sec18p and NSF.

Immunolocalization and partial characterization of a nucleolar autoantigen (PM-Scl) associated with polymyositis/scleroderma overlap syndromes.