Publications David Haselbach | Scientific Papers Haselbach Lab

Zhang, Y., Grundmann, L., Vollmar, L., Schimpf, J., Hübscher, V., Areeb, M., Grishkovskaya, I., Moddemann, A., Werner, K., Hugel, T., Haselbach, D., Rospert, S. (2026)

The cotranslational cycle of the ribosome-bound Hsp70 homolog Ssb.

Nat Commun. 17(1):961

Gerhalter, M., Prattes, M., Grundmann, LE., Grishkovskaya, I., Semeraro, EF., Zisser, G., Kotisch, H., Merl-Pham, J., Hauck, SM., Haselbach, D., Bergler, H. (2026)

A comprehensive view on r-protein binding and rRNA domain structuring during early eukaryotic ribosome formation.

Nucleic Acids Res. 54(3)

Brunner, HL., Kalis, RW., Grundmann, L., Hodáková, Z., Koskova, Z., Grishkovskaya, I., de Almeida, M., Hinterndorfer, M., Knaudt, H., Höfflin, S., Andersch, F., Kotisch, H., Dickmanns, A., Cuylen-Haering, S., Zuber, J., Haselbach, D. (2026)

A multivalent adaptor mechanism drives the nuclear import of proteasomes.

Nat Commun.

Schwartz, I., Budroni, V., Meyenberg, M., Hodakova, Z., Hornegger, H., Hacker, K., Schwartz, S., Grabarczyk, DB., Ehrmann, JF., Scinicariello, S., Haselbach, D., Menche, J., Clausen, T., Karagöz, GE., Versteeg, GA. (2026)

Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity.

Nat Commun.

de Araujo, MEG., J Amann, S., Stasyk, T., Schleiffer, A., Rauch, E., Flümann, P., Singer, II., Kremser, L., Dostal, V., Laopanupong, T., Obojes, N., Wallnöfer, MH., Gradl, FS., Kurzbauer, R., Krebiehl, C., Kofler, S., Grishkovskaya, I., Vogel, GF., Hess, MW., Sarg, B., Clausen, T., Haselbach, D., Huber, LA. (2026)

BORC assemblies integrate BLOC-1 subunits to diversify endosomal trafficking functions.

Proc Natl Acad Sci U S A. 123(4):e2515691123

De la Concepcion, JC., Duverge, H., Kim, Y., Julian, J., Xu, HD., Watt, MN., Ikene, SA., Bianchi, A., Grujic, N., Papareddy, RK., Grishkovskaya, I., Haselbach, D., Murray, DH., Clavel, M., Irwin, NAT., Dagdas, Y. (2025)

Electrostatic changes enabled the diversification of an exocyst subunit via protein complex escape.

Nat Plants. 11(11):2350-2367

Harar, P., Herrmann, L., Grohs, P., Haselbach, D. (2025)

FakET: Simulating cryo-electron tomograms with neural style transfer.

Structure. 33(4):820-827.e4

Rudi, O., Hodakova, Z., Farias Saad, C., Winter, N., Grishkovskaya, I., Böhm, J., Jarck, G., Schleiffer, A., Haselbach, D., Bachmair, A. (2025)

The UBR Domain of Plant Ubr1 Homolog PRT6 Accommodates Basic and Hydrophobic Amino Termini for Substrate Recognition.

J Mol Biol. 437(4):168939

Husremović, T., Meier, V., Piëch, L., Siess, KM., Antonioli, S., Grishkovskaya, I., Kircheva, N., Angelova, SE., Wenzl, K., Brandstätter, A., Veis, J., Miočić-Stošić, F., Anrather, D., Hartl, M., Truebestein, L., Cerron-Alvan, LM., Leeb, M., Žagrović, B., Hann, S., Bock, C., Ogris, E., Dudev, T., Irwin, NAT., Haselbach, D., Leonard, TA. (2025)

PHLPP2 is a pseudophosphatase that lost activity in the metazoan ancestor.

Proc Natl Acad Sci U S A. 122(14):e2417218122

Robertson, KC., Amann, SJ., Liu, T., Funk, AV., Wang, X., Grishkovskaya, I., Tabor, JR., Norris-Drouin, JL., Arrowsmith, CH., Collins, JL., Emanuele, MJ., Haselbach, D., James, LI., Brown, NG. (2025)

Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22.

bioRxiv.

Pre-printBrunner HL, Kalis RW, Grundmann L, Hodáková Z, Koskova Z, Grishkovskaya I, de Almeida M, Hinterndorfer M, Höfflin S, Andersch F, Kotisch H, Dickmanns A, Cuylen-Häring S, Zuber J, Haselbach D (2024)

A multivalent adaptor mechanism drives the nuclear import of proteasomes

625870.

Kofler, L., Grundmann, L., Gerhalter, M., Prattes, M., Merl-Pham, J., Zisser, G., Grishkovskaya, I., Hodirnau, VV., Vareka, M., Breinbauer, R., Hauck, SM., Haselbach, D., Bergler, H. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Nat Commun. 15(1):7511

Pre-printLambrecht F, Kröpelin A, Lüttich M, Habeck M, Haselbach D, Stark H (2024)

CowScape: Quantitative reconstruction of the conformational landscape of biological macromolecules from cryo-EM data

arXiv. 2402.11589

Pre-printDe La Concepcion JC, Duverge H, Kim Y, Julian J, Xu HD, Ikene SA, Bianchi A, Grujic N, Papareddy RK, Grishkovskaya I, Haselbach D, Murray DH, Clavel M , Irwin NAT, Dagdas Y (2024)

Electrostatic changes enabled the diversification of an exocyst subunit via protein complex escape

bioRxiv. 2024.08.26.609756

Pre-printSchwartz I, Budroni V, Meyenberg M, Hornegger H, Hacker K, Schwartz S, Hodakova Z, Grabarczyk DB, Ehrmann JF , Scinicariello S, Haselbach D, Menche J, Clausen T, Karagöz E, Versteeg GA (2024)

Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity

bioRxiv. 2024.04.23.590688

Pre-printShah, AN., Leesch, F., Lorenzo-Orts, L., Grundmann, L., Novatchkova, M., Haselbach, D., Calo, E., Pauli, A. (2024)

A dual ribosomal system in the zebrafish soma and germline

bioRxiv. doi:10.1101/2024.08.29.610041

Hodáková, Z., Grishkovskaya, I., Brunner, HL., Bolhuis, DL., Belačić, K., Schleiffer, A., Kotisch, H., Brown, NG., Haselbach, D. (2023)

Cryo-EM structure of the chain-elongating E3 ubiquitin ligase UBR5.

EMBO J. 42(16):e113348

Bodrug, T., Welsh, KA., Bolhuis, DL., Paulаkonis, E., Martinez-Chacin, RC., Liu, B., Pinkin, N., Bonacci, T., Cui, L., Xu, P., Roscow, O., Amann, SJ., Grishkovskaya, I., Emanuele, MJ., Harrison, JS., Steimel, JP., Hahn, KM., Zhang, W., Zhong, ED., Haselbach, D., Brown, NG. (2023)

Time-resolved cryo-EM (TR-EM) analysis of substrate polyubiquitination by the RING E3 anaphase-promoting complex/cyclosome (APC/C).

Nat Struct Mol Biol. 30(11):1663-1674

Amann, SJ., Keihsler, D., Bodrug, T., Brown, NG., Haselbach, D. (2023)

Frozen in time: analyzing molecular dynamics with time-resolved cryo-EM.

Structure. 31(1):4-19

Leesch, F., Lorenzo-Orts, L., Pribitzer, C., Grishkovskaya, I., Roehsner, J., Chugunova, A., Matzinger, M., Roitinger, E., Belačić, K., Kandolf, S., Lin, TY., Mechtler, K., Meinhart, A., Haselbach, D., Pauli, A. (2023)

A molecular network of conserved factors keeps ribosomes dormant in the egg.

Corresponding preprint Nature.

Pechincha, C., Groessl, S., Kalis, R., de Almeida, M., Zanotti, A., Wittmann, M., Schneider, M., de Campos, RP., Rieser, S., Brandstetter, M., Schleiffer, A., Müller-Decker, K., Helm, D., Jabs, S., Haselbach, D., Lemberg, MK., Zuber, J., Palm, W. (2022)

Lysosomal enzyme trafficking factor LYSET enables nutritional usage of extracellular proteins.

Science. 378(6615):eabn5637

Kandolf, S., Grishkovskaya, I., Belačić, K., Bolhuis, DL., Amann, S., Foster, B., Imre, R., Mechtler, K., Schleiffer, A., Tagare, HD., Zhong, ED., Meinhart, A., Brown, NG., Haselbach, D. (2022)

Cryo-EM structure of the plant 26S proteasome.

Plant Commun. 3(3):100310

Prattes, M., Grishkovskaya, I., Hodirnau, VV., Hetzmannseder, C., Zisser, G., Sailer, C., Kargas, V., Loibl, M., Gerhalter, M., Kofler, L., Warren, AJ., Stengel, F., Haselbach, D., Bergler, H. (2022)

Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1.

Nat Struct Mol Biol. 29(9):942-953

Morreale, FE., Kleine, S., Leodolter, J., Junker, S., Hoi, DM., Ovchinnikov, S., Okun, A., Kley, J., Kurzbauer, R., Junk, L., Guha, S., Podlesainski, D., Kazmaier, U., Boehmelt, G., Weinstabl, H., Rumpel, K., Schmiedel, VM., Hartl, M., Haselbach, D., Meinhart, A., Kaiser, M., Clausen, T. (2022)

BacPROTACs mediate targeted protein degradation in bacteria.

Corresponding preprint Cell. 185(13):2338-2353.e18

Schimpf, J., Oppermann, S., Gerasimova, T., Santos Seica, AF., Hellwig, P., Grishkovskaya, I., Wohlwend, D., Haselbach, D., Friedrich, T. (2021)

Structure of the peripheral arm of a minimalistic respiratory complex I.

Structure.

Gutiérrez-Pérez, P., Santillán, EM., Lendl, T., Wang, J., Schrempf, A., Steinacker, TL., Asparuhova, M., Brandstetter, M., Haselbach, D., Cochella, L. (2021)

miR-1 sustains muscle physiology by controlling V-ATPase complex assembly.

Sci Adv. 7(42):eabh1434

de Almeida, M., Hinterndorfer, M., Brunner, H., Grishkovskaya, I., Singh, K., Schleiffer, A., Jude, J., Deswal, S., Kalis, R., Vunjak, M., Lendl, T., Imre, R., Roitinger, E., Neumann, T., Kandolf, S., Schutzbier, M., Mechtler, K., Versteeg, GA., Haselbach, D., Zuber, J. (2021)

AKIRIN2 controls the nuclear import of proteasomes in vertebrates.

Nature. 599(7885):491-496

Grabarczyk, DB., Petrova, OA., Deszcz, L., Kurzbauer, R., Murphy, P., Ahel, J., Vogel, A., Gogova, R., Faas, V., Kordic, D., Schleiffer, A., Meinhart, A., Imre, R., Lehner, A., Neuhold, J., Bader, G., Stolt-Bergner, P., Böttcher, J., Wolkerstorfer, B., Fischer, G., Grishkovskaya, I., Haselbach, D., Kessler, D., Clausen, T. (2021)

HUWE1 employs a giant substrate-binding ring to feed and regulate its HECT E3 domain.

Nat Chem Biol.

Prattes, M., Grishkovskaya, I., Hodirnau, VV., Rössler, I., Klein, I., Hetzmannseder, C., Zisser, G., Gruber, CC., Gruber, K., Haselbach, D., Bergler, H. (2021)

Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine.

Nat Commun. 12(1):3483

Rodriguez Carvajal, A., Grishkovskaya, I., Gomez Diaz, C., Vogel, A., Sonn-Segev, A., Kushwah, MS., Schodl, K., Deszcz, L., Orban-Nemeth, Z., Sakamoto, S., Mechtler, K., Kukura, P., Clausen, T., Haselbach, D., Ikeda, F. (2021)

The linear ubiquitin chain assembly complex (LUBAC) generates heterotypic ubiquitin chains.

Corresponding preprint Elife. 10

Sonn-Segev, A., Belacic, K., Bodrug, T., Young, G., VanderLinden, RT., Schulman, BA., Schimpf, J., Friedrich, T., Dip, PV., Schwartz, TU., Bauer, B., Peters, JM., Struwe, WB., Benesch, JLP., Brown, NG., Haselbach, D., Kukura, P. (2020)

Quantifying the heterogeneity of macromolecular machines by mass photometry.

Nat Commun. 11(1):1772

Ahel, J., Lehner, A., Vogel, A., Schleiffer, A., Meinhart, A., Haselbach, D., Clausen, T. (2020)

Moyamoya disease factor RNF213 is a giant E3 ligase with a dynein-like core and a distinct ubiquitin-transfer mechanism.

Elife. 9

Prajapati, S., Haselbach, D., Wittig, S., Patel, MS., Chari, A., Schmidt, C., Stark, H., Tittmann, K. (2019)

Structural and Functional Analyses of the Human PDH Complex Suggest a "Division-of-Labor" Mechanism by Local E1 and E3 Clusters.

Structure. 27(7):1124-1136.e4

Watson, ER., Grace, CRR., Zhang, W., Miller, DJ., Davidson, IF., Prabu, JR., Yu, S., Bolhuis, DL., Kulko, ET., Vollrath, R., Haselbach, D., Stark, H., Peters, JM., Brown, NG., Sidhu, SS., Schulman, BA. (2019)

Protein engineering of a ubiquitin-variant inhibitor of APC/C identifies a cryptic K48 ubiquitin chain binding site.

Proc Natl Acad Sci U S A. 116(35):17280-17289

Haselbach, D., Komarov, I., Agafonov, DE., Hartmuth, K., Graf, B., Dybkov, O., Urlaub, H., Kastner, B., Lührmann, R., Stark, H. (2018)

Structure and Conformational Dynamics of the Human Spliceosomal Bact Complex.

Cell. 172(3):454-464.e11

Haselbach, D., Schrader, J., Lambrecht, F., Henneberg, F., Chari, A., Stark, H. (2017)

Long-range allosteric regulation of the human 26S proteasome by 20S proteasome-targeting cancer drugs.

Nat Commun. 8:15578

Bertram, K., Agafonov, DE., Dybkov, O., Haselbach, D., Leelaram, MN., Will, CL., Urlaub, H., Kastner, B., Lührmann, R., Stark, H. (2017)

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.

Cell. 170(4):701-713.e11

Qiao, R., Weissmann, F., Yamaguchi, M., Brown, NG., VanderLinden, R., Imre, R., Jarvis, MA., Brunner, MR., Davidson, IF., Litos, G., Haselbach, D., Mechtler, K., Stark, H., Schulman, BA., Peters, JM. (2016)

Mechanism of APC/CCDC20 activation by mitotic phosphorylation.

Proc Natl Acad Sci U S A. 113(19):E2570-8

Brown, NG., VanderLinden, R., Watson, ER., Weissmann, F., Ordureau, A., Wu, KP., Zhang, W., Yu, S., Mercredi, PY., Harrison, JS., Davidson, IF., Qiao, R., Lu, Y., Dube, P., Brunner, MR., Grace, CRR., Miller, DJ., Haselbach, D., Jarvis, MA., Yamaguchi, M., Yanishevski, D., Petzold, G., Sidhu, SS., Kuhlman, B., Kirschner, MW., Harper, JW., Peters, JM., Stark, H., Schulman, BA. (2016)

Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C.

Cell. 165(6):1440-1453

Yamaguchi, M., VanderLinden, R., Weissmann, F., Qiao, R., Dube, P., Brown, NG., Haselbach, D., Zhang, W., Sidhu, SS., Peters, JM., Stark, H., Schulman, BA. (2016)

Cryo-EM of Mitotic Checkpoint Complex-Bound APC/C Reveals Reciprocal and Conformational Regulation of Ubiquitin Ligation.

Mol Cell. 63(4):593-607

Chari, A., Haselbach, D., Kirves, JM., Ohmer, J., Paknia, E., Fischer, N., Ganichkin, O., Möller, V., Frye, JJ., Petzold, G., Jarvis, M., Tietzel, M., Grimm, C., Peters, JM., Schulman, BA., Tittmann, K., Markl, J., Fischer, U., Stark, H. (2015)

ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space.

Nat Methods. 12(9):859-65

Monecke, T., Haselbach, D., Voß, B., Russek, A., Neumann, P., Thomson, E., Hurt, E., Zachariae, U., Stark, H., Grubmüller, H., Dickmanns, A., Ficner, R. (2013)

Structural basis for cooperativity of CRM1 export complex formation.

Proc Natl Acad Sci U S A. 110(3):960-5

Dölker, N., Blanchet, CE., Voß, B., Haselbach, D., Kappel, C., Monecke, T., Svergun, DI., Stark, H., Ficner, R., Zachariae, U., Grubmüller, H., Dickmanns, A. (2013)

Structural determinants and mechanism of mammalian CRM1 allostery.

Structure. 21(8):1350-60

Köster, DM., Haselbach, D., Lehrach, H., Seitz, H. (2011)

A DNAzyme based label-free detection system for miniaturized assays.

Mol Biosyst. 7(10):2882-9

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