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Clemens Plaschka

Messenger RNA (mRNA) maturation is a crucial step in gene expression, requiring the coordinated action of several macromolecular complexes. The most dramatic maturation event is the removal of introns from pre-mRNA by the spliceosome, a large and dynamic ribonucleoprotein enzyme. We apply structural and functional methods to elucidate the molecular basis of spliceosome regulation and its coordination with other maturation events. Our goal is to obtain snapshots of the different stages of mRNA maturation to understand how a functional mRNA is produced.

Publications

Wilkinson, ME., Lin, PC., Plaschka, C., Nagai, K. (2018) Cryo-EM Studies of Pre-mRNA Splicing: From Sample Preparation to Model Visualization. Annu Rev Biophys.
Plaschka, C., Lin, PC., Nagai, K. (2017) Structure of a pre-catalytic spliceosome. Nature. 546(7660):617-621
Plaschka, C., Hantsche, M., Dienemann, C., Burzinski, C., Plitzko, J., Cramer, P. (2016) Transcription initiation complex structures elucidate DNA opening. Nature. 533(7603):353-8
Plaschka, C., Nozawa, K., Cramer, P. (2016) Mediator Architecture and RNA Polymerase II Interaction. J Mol Biol. 428(12):2569-2574
Plaschka, C., Larivière, L., Wenzeck, L., Seizl, M., Hemann, M., Tegunov, D., Petrotchenko, EV., Borchers, CH., Baumeister, W., Herzog, F., Villa, E., Cramer, P. (2015) Architecture of the RNA polymerase II-Mediator core initiation complex. Nature. 518(7539):376-80
Larivière, L., Plaschka, C., Seizl, M., Petrotchenko, EV., Wenzeck, L., Borchers, CH., Cramer, P. (2013) Model of the Mediator middle module based on protein cross-linking. Nucleic Acids Res. 41(20):9266-73
Larivière, L., Plaschka, C., Seizl, M., Wenzeck, L., Kurth, F., Cramer, P. (2012) Structure of the Mediator head module. Nature. 492(7429):448-51