BioOptics Department


Head: Karin Aumayr  

 

The services offered by the BioOptics Facility to researchers at IMP, IMBA and GMI cover analytical flow cytometry and cell sorting, as well as a large variety of microscopy techniques, image processing and analysis.

Flow Cytometry

Figure (Click to view legend)

We provide instrumentation, education and expertise for all flow cytometry requiring experiments, and offer high-speed cell sorting as well as advanced and general flow cytometry analysis. Users are given guidance and support with the planning of experiments and implementation of new fluorophore combinations. They are trained in the use of hardware and software for all of the three available state-of-the-art flow cytometers, as well as in data processing and analysis. Two cell sorters are operated by the staff of the facility to sort up to four cell populations simultaneously in bulk, or conduct single cell sorting.

Microscopy

The BioOptics Facility currently manages more than twenty microcopy systems, covering widefield microscopy, confocal laser scanning microscopy (CLSM), two-photon (2P) microscopy, total internal reflection (TIRF) microscopy techniques, and an automated slide scanner for samples with or without fluorescence. Most of the systems are motorized, thus providing automation for higher throughput, and are suitable for both fixed samples and live cell experiments. The facility provides assisted use and training on all instrumentation and consultation concerning all microscopy-related subjects, including project planning, staining, microscope selection, etc.

Image Processing and Analysis

Five state-of-the-art computer workstations are available at the BioOptics Facility, operating most of the common commercial image processing and visualization software. For the deconvolution of microscopy images, a server solution with a web-based interface has been set up in order to provide efficient, multi-user, parallel, batch deconvolution that can easily be started from the individual scientist’s computer. Users are trained in the use of specific software, depending on their demands. Several image analysis algorithms are available, such as object tracking and tracing, determination of measurement parameters like intensity, distance, area, volume and co-localization. For advanced image analysis and automated object recognition, customized classification and measuring algorithms are developed within the facility.
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