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David Keays

The Keays lab aims to understand two biological phenomena: 1) How do neurons migrate in the developing brain? and 2) How do animals detect magnetic fields? In tackling these two questions we adopt an interdisciplinary approach, employing a wide range of genetic, molecular, cellular, and behavioural tools.

Neuronal migration and magnetoreception

Tubulins in neuronal migration and disease

Figure 1: The Jenna mutant mouse. This mouse harbours a S140G mutation in the Tuba1a gene, which results in defective neuronal migration during development. As a result, the Jenna mouse is characterised by abnormal lamination of the hippocampus, accompanied by hyperactivity and deficits in cognitive tasks.

Neuronal migration underlies the organization of the mammalian brain. All neurons that are born in the proliferative ventricular zones migrate to their final destination by extending their primary neurites and translocating their nuclei. This migration is crucial because it not only determines the destination of a given neuron, but also the circuit in which it operates. Moreover, a host of neurodevelopmental diseases are known to be linked with defective neuronal migration. One example is lissencephaly, a disease that is characterized by a “smooth brain”, epilepsy, and mental retardation. We have shown that mutations in the alpha tubulin gene TUBA1A cause lissencephaly in humans, and neuronal migration abnormalities in mice (Keays et al., 2007) (Figure 1). The importance of the tubulin gene family in cortical development is further illustrated by our finding that mutations in the beta tubulin gene TUBB2B cause polymicrogyria, a disease characterized by excessive folding of the cortex (Jalgin et al., 2009). Most recently we have shown that mutations in TUBB5 cause a reduction in brain size in humans, and perturb both migration and neurogenic division in mice (Breuss et al., 2012).

To gain insight into the role of different tubulin genes, how they cause disease, and the molecular mechanisms underlying the migration of neurons, the Keays lab is employing the mouse as a model system. We use ENU mutagenesis, as well as transgenic methods to generate new models for human disease. To complement these murine studies, we are taking advantage of next-generation sequencing, and in collaboration with a network of clinical colleagues, sequencing the exomes of patients with sporadic neuronal migration disorders. These genetic studies have already identified a number of new disease-causing genes which are currently being functionally interrogated.

Molecular and cellular basis of magnetoreception

Figure 2: Pigeons; our model system for studying how animals detect the Earth’s magnetic field.

Many species on the planet, whether they are birds, fish or insects, rely on the Earth’s magnetic field to guide migration or assist navigation. This remarkable sense is known as magnetoreception. One idea that aims to explain how animals detect magnetic fields is known as the magnetite-based theory of magnetoreception. This theory holds that mechanosensitive ion channels coupled to an intracellular compass made of an iron oxide known as magnetite (Fe3O4) transduce local magnetic information into a neuronal impulse.

We are using the rock pigeon Columbia livia as a model (Figure 2). Previous studies have asserted that pigeons employ a magnetite-containing sensory apparatus located at six specific loci in the skin of the beak. We have shown that this established dogma is false, and in reality clusters of iron-rich cells in the beak of pigeons are macrophages; they are not magnetosensitive neurons (Treiber, Salzer et al., 2012). The field is now engaged in a renewed search for the true magnetoreceptive cells. Where are the magnetosensory cells in avian species?

Figure 3: Pigeon hair cells stained with Prussian blue (which labels ferric iron) and nuclear fast red. A single iron-rich organelle is found per cell, and is located beneath the stereocilia. The scale bar shows 10 μm.

Recent electrophysiological studies have identified a population of neurons in the vestibular nuclei of pigeons that respond to magnetic vectors of a specific orientation and intensity (Wu and Dickman, 2012). These results strongly suggest that a population of unidentified magnetosensory cells lie in the inner ear of pigeons. Employing histological techniques, we identified sensory hair cells that contain a single iron-rich organelle (Figure 3). This organelle is located directly beneath the stereocilia and is embedded in the actin-rich cuticular plate. We have shown that this structure is present in a wide range of avian species, but is not found in rodents, fish, or humans (Lauwers, Pichler et al., 2013). Our current work is focused on elucidating the function of this organelle, specifically whether it plays a direct or indirect role in magnetoreception. 

Collaborators

Professor Nick Cowan (NYU, New York) – Tubulin biochemistry
Professor Jamel Chelly (Inserm, Paris) – Tubulin and lisssencephaly
Professor Jonathan Flint (Oxford, UK) – Neurodevelopmental mouse mutants

Selected Publications

  • Edelman, NB., Fritz, T., Nimpf, S., Pichler, P., Lauwers, M., Hickman, RW., Papadaki-Anastasopoulou, A., Ushakova, L., Heuser, T., Resch, GP., Saunders, M., Shaw, JA., Keays, DA. (2015). No evidence for intracellular magnetite in putative vertebrate magnetoreceptors identified by magnetic screening. Proc Natl Acad Sci U S A. 112(1):262-7 (abstract)
  • Lauwers, M., Pichler, P., Edelman, NB., Resch, GP., Ushakova, L., Salzer, MC., Heyers, D., Saunders, M., Shaw, J., Keays, DA. (2013). An iron-rich organelle in the cuticular plate of avian hair cells. Curr Biol. 23(10):924-9 
  • Breuss, M., Heng, JI., Poirier, K., Tian, G., Jaglin, XH., Qu, Z., Braun, A., Gstrein, T., Ngo, L., Haas, M., Bahi-Buisson, N., Moutard, ML., Passemard, S., Verloes, A., Gressens, P., Xie, Y., Robson, KJ., Rani, DS., Thangaraj, K., Clausen, T., Chelly, J., Cowan, NJ., Keays, DA. (2012). Mutations in the β-tubulin gene TUBB5 cause microcephaly with structural brain abnormalities. Cell Rep. 2(6):1554-62 
  • Treiber, CD., Salzer, MC., Riegler, J., Edelman, N., Sugar, C., Breuss, M., Pichler, P., Cadiou, H., Saunders, M., Lythgoe, M., Shaw, J., Keays, DA. (2012). Clusters of iron-rich cells in the upper beak of pigeons are macrophages not magnetosensitive neurons. Nature. 484(7394):367-70 
  • Keays, DA., Tian, G., Poirier, K., Huang, GJ., Siebold, C., Cleak, J., Oliver, PL., Fray, M., Harvey, RJ., Molnár, Z., Piñon, MC., Dear, N., Valdar, W., Brown, SD., Davies, KE., Rawlins, JN., Cowan, NJ., Nolan, P., Chelly, J., Flint, J. (2007). Mutations in alpha-tubulin cause abnormal neuronal migration in mice and lissencephaly in humans. Cell. 128(1):45-57

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