Mass Spectrometry & Protein Chemistry
Karl Mechtler (Head)
Mass spectrometry (MS) has emerged as a core analytical technique in protein chemistry
Driven by the rapid development of instrumentation, analysis methods and computing tools, MS based proteomics is at the forefront of techniques in modern life sciene research.
In the Mechtler lab, we are interested in developing new methods to increase the sensitivity, accuracy and precision of protein identification/quantification and the detection of post translational modifications. Our aim is to use these optimized methods to answer fundamental biological questions.
Selected Publications
- Hutchins, JR., Toyoda, Y., Hegemann, B., Poser, I., Hériché, JK., Sykora, MM., Augsburg, M., Hudecz, O., Buschhorn, BA., Bulkescher, J., Conrad, C., Comartin, D., Schleiffer, A., Sarov, M., Pozniakovsky, A., Slabicki, MM., Schloissnig, S., Steinmacher, I., Leuschner, M., Ssykor, A., Lawo, S., Pelletier, L., Stark, H., Nasmyth, K., Ellenberg, J., Durbin, R., Buchholz, F., Mechtler, K., Hyman, AA., Peters, JM. (2010). Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science. 328(5978):593-9 (abstract)
- Leitner, A., Sturm, M., Hudecz, O., Mazanek, M., Smått, JH., Lindén, M., Lindner, W., Mechtler, K. (2010). Probing the phosphoproteome of HeLa cells using nanocast metal oxide microspheres for phosphopeptide enrichment. Anal Chem. 82(7):2726-33 (abstract)
- Pichler, P., Köcher, T., Holzmann, J., Mazanek, M., Taus, T., Ammerer, G., Mechtler, K. (2010). Peptide labeling with isobaric tags yields higher identification rates using iTRAQ 4-plex compared to TMT 6-plex and iTRAQ 8-plex on LTQ Orbitrap. Anal Chem. 82(15):6549-58 (abstract)
- Holzmann, J., Pichler, P., Madalinski, M., Kurzbauer, R., Mechtler, K. (2009). Stoichiometry determination of the MP1-p14 complex using a novel and cost-efficient method to produce an equimolar mixture of standard peptides. Anal Chem. 81(24):10254-61 (abstract)
- Köcher, T., Pichler, P., Schutzbier, M., Stingl, C., Kaul, A., Teucher, N., Hasenfuss, G., Penninger, JM., Mechtler, K. (2009). High precision quantitative proteomics using iTRAQ on an LTQ Orbitrap: a new mass spectrometric method combining the benefits of all. J Proteome Res. 8(10):4743-52 (abstract)
