Mass Spectrometry & Protein Chemistry

Karl Mechtler (Head)

(Email)

Mass spectrometry (MS) has emerged as a core analytical technique in protein chemistry

Driven by the rapid development of instrumentation, analysis methods and computing tools, MS based proteomics is at the forefront of techniques in modern life science research. 

In the Mechtler lab, we are interested in developing new methods to increase the sensitivity, accuracy and precision of protein identification/quantification and the detection of post translational modifications. Our aim is to use these optimized methods to answer fundamental biological questions.

Selected Publications

  • Trentini, DB., Suskiewicz, MJ., Heuck, A., Kurzbauer, R., Deszcz, L., Mechtler, K., Clausen, T. (2016). Arginine phosphorylation marks proteins for degradation by a Clp protease. Nature. 539(7627):48-53 (abstract)
  • Rampler, E., Stranzl, T., Orban-Nemeth, Z., Hollenstein, DM., Hudecz, O., Schloegelhofer, P., Mechtler, K. (2015). Comprehensive Cross-Linking Mass Spectrometry Reveals Parallel Orientation and Flexible Conformations of Plant HOP2-MND1. J Proteome Res. 14(12):5048-62 (abstract)
  • Roitinger, E., Hofer, M., Köcher, T., Pichler, P., Novatchkova, M., Yang, J., Schlögelhofer, P., Mechtler, K. (2015). Quantitative phosphoproteomics of the ataxia telangiectasia-mutated (ATM) and ataxia telangiectasia-mutated and rad3-related (ATR) dependent DNA damage response in Arabidopsis thaliana. Mol Cell Proteomics. 14(3):556-71 (abstract)
  • Dorfer, V., Pichler, P., Stranzl, T., Stadlmann, J., Taus, T., Winkler, S., Mechtler, K. (2014). MS Amanda, a universal identification algorithm optimized for high accuracy tandem mass spectra. J Proteome Res. 13(8):3679-84 (abstract)
  • Jüschke, C., Dohnal, I., Pichler, P., Harzer, H., Swart, R., Ammerer, G., Mechtler, K., Knoblich, JA. (2013). Transcriptome and proteome quantification of a tumor model provides novel insights into post-transcriptional gene regulation. Genome Biol. 14(11):r133 (abstract)

Grants