The Histology Service Department offers state-of-the art sectioning, histology and immunohistochemistry services to all IMBA and IMP scientists. In addition, we offer training and support for researchers in new technologies.
Histology Services
Figure 1 (Click to view legend)
The histology services include the embedding of tissues, assistance with the sectioning of paraffin and cryo-preserved tissues, and preparation of slides for standard H&E stains, as well as specialized stainings such as PAS, Alcian blue, Cab, Gomeri, MayGruenwald-Giemsa and van Kossa stains for human, mouse, Xenopus and Drosophila studies. With these services, we are able to offer support to get quick results.
Sectioning of Paraffin and Frozen Tissues
In our group we have developed a high throughput method to cut paraffin and frozen tissues. Using this method, we could increase the quality and also the quantity of services.
Immunohistochemistry
Figure 2 (Click to view legend)
The Histology Service Department also provides automated preparation and processing facilities for standardized immuno-histochemistry, in situ hybridization, FISH analysis, DNA microarray and tissues microarray applications. Various antibodies have been characterized for optimized in situ signaling studies. Standard antibodies such as apc, cd (several markers), I-ad, gfp, gfap, c-fos, c-jun, junB, fra1,2, jun-D, ki67, smad3, brdu, egf, egfr, H3K9me tri meth, H4K20me3 tri meth, cl. caspase3, caspase7, procatepsiK are available. In addition, the Histology Service Department administrates legal regulatory affairs such as the record-keeping and documentation of experiments in accordance with the Austrian Histolaboratories guidelines (www.mta-labor.info).
Selected Publications
2008
Bozec, A., Bakiri, L., Hoebertz, A., Eferl, R., Schilling, AF., Komnenovic, V., Scheuch, H., Priemel, M., Stewart, CL., Amling, M., Wagner, EF. (2008). Osteoclast size is controlled by Fra-2 through LIF/LIF-receptor signalling and hypoxia.
Nature. 454(7201):221-5
(abstract)
2007
Andrade, N., Komnenovic, V., Blake, SM., Jossin, Y., Howell, B., Goffinet, A., Schneider, WJ., Nimpf, J. (2007). ApoER2/VLDL receptor and Dab1 in the rostral migratory stream function in postnatal neuronal migration independently of Reelin.
Proc Natl Acad Sci U S A. 104(20):8508-13
(abstract)
Hui, L., Bakiri, L., Mairhorfer, A., Schweifer, N., Haslinger, C., Kenner, L., Komnenovic, V., Scheuch, H., Beug, H., Wagner, EF. (2007). p38alpha suppresses normal and cancer cell proliferation by antagonizing the JNK-c-Jun pathway.
Nat Genet. 39(6):741-9
(abstract)
Kuba, K., Zhang, L., Imai, Y., Arab, S., Chen, M., Maekawa, Y., Leschnik, M., Leibbrandt, A., Markovic, M., Makovic, M., Schwaighofer, J., Beetz, N., Musialek, R., Neely, GG., Komnenovic, V., Kolm, U., Metzler, B., Ricci, R., Hara, H., Meixner, A., Nghiem, M., Chen, X., Dawood, F., Wong, KM., Sarao, R., Cukerman, E., Kimura, A., Hein, L., Thalhammer, J., Liu, PP., Penninger, JM. (2007). Impaired heart contractility in Apelin gene-deficient mice associated with aging and pressure overload.
Circ Res. 101(4):e32-42
(abstract)
2006
Jones, DH., Nakashima, T., Sanchez, OH., Kozieradzki, I., Komarova, SV., Sarosi, I., Morony, S., Rubin, E., Sarao, R., Hojilla, CV., Komnenovic, V., Kong, YY., Schreiber, M., Dixon, SJ., Sims, SM., Khokha, R., Wada, T., Penninger, JM. (2006). Regulation of cancer cell migration and bone metastasis by RANKL.
Nature. 440(7084):692-6
(abstract)
